The structural biology of trace metal trafficking across membranes

痕量金属跨膜运输的结构生物学

• 批准号: FT180100397
• 负责人: Prof Megan Maher
• 金额: $ 61.59万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-03-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT180100397
• 关键词: structural; biology; trace; metal; trafficking

This project aims to investigate how essential trace element nutrients are recognised and specifically acquired and expelled by bacterial cells. Cells are surrounded by biomembranes that separate and protect them from their environments. Embedded within these membranes are proteins that perform essential functions. In bacteria, membrane proteins are responsible for the uptake and elimination of trace elements that are required for survival. This project will investigate the features of integral membrane proteins that allow discrimination between cargo, by defining their three dimensional architectures using X-ray crystallography. This will contribute to the field of membrane protein structural biology and fundamental discoveries in the area of cellular trace element homeostasis and toxicity.

该项目旨在研究细菌细胞如何识别和特异性获取和排出必需的微量元素营养素。细胞被生物膜包围，生物膜将细胞与环境隔离并保护细胞。嵌入在这些膜内的是执行基本功能的蛋白质。在细菌中，膜蛋白负责吸收和消除生存所需的微量元素。该项目将研究整体膜蛋白的特征，通过使用x射线晶体学定义其三维结构来区分货物。这将有助于膜蛋白结构生物学领域和细胞微量元素稳态和毒性领域的基础性发现。