STRUCTURE/FUNCTION COMPARISONS OF HIV 1 & RSV PROTEASES

描述(改编自调查者摘要)：结构 通过对RSV和HIV-1的结晶学研究获得的信息 蛋白水解酶将指导目前的研究，试图检查其作用 决定底物选择的关键残基和 催化效率。试图将一种公关的特殊性改变为 对于异源和同源底物的另一种将是 通过定点突变和生化检测进行 纯化酶的特性。PR亚单位对称性的影响 为了获得最佳的催化效率，将通过选择 PR同源二聚体的个别亚基的突变。这些信息 从这些实验中收集到的信息将被用来构建一个 蛋白酶“作用于HIV-1RT上正常靶点以外的位置。 最后，改变后的蛋白酶将在活体内进行测试，以便进一步 了解它们在涉及病毒的生物过程中的作用 组装和繁殖。

DESCRIPTION (Adapted from investigator's abstract): The structural information obtained through crystallographic studies on RSV and HIV-1 proteases will guide the present study in attempts to examine the role of critical residues that determine the substrate selection and catalytic efficiency. Attempts to change the specificity of one PR into the other for both heterologous and homologous substrates will be undertaken by site-directed mutagenesis and tested by biochemical characterization of purified enzymes. The effect of PR subunit symmetry for optimal catalytic efficiencies will be examined by selective mutagenesis of individual subunits of the PR homodimer. The information gathered from these experiments would be used to construct a "designer protease" to act on other than normal target sites on HIV-1 RT. Finally, altered proteases will be tested in vivo , for further understanding of their role in biological processes involved in virus assembly and reproduction.