MUTATION AND RECOMBINATION OF RETROVIRUSES

描述：张博士建议比较体内的突变率 在第一和第二链DNA合成过程中。他将利用一种体外培养技术 比较RNA聚合酶II、RT第一链、 RT第二链合成。他将测试三种流行的型号 体内和体外逆转录病毒的重组是如何发生的 系统。他还将测试RNA二级结构对 重组的刺激。

DESCRIPTION: Dr. Zhang proposes to compare the rate of mutation in vivo during first and second strand DNA synthesis. He will utilize an in vitro assay to compare the frameshift rates of RNA polymerase II, RT first strand, and RT second strand synthesis. He will test the three prevailing models for how recombination occurs in retroviruses using in vivo and in vitro systems. He will also test the effects of RNA secondary structure on stimulation of recombination.