MUTATION AND RECOMBINATION OF RETROVIRUSES

逆转录病毒的突变和重组

• 批准号: 2895526
• 负责人: JIAYOU ZHANG
• 金额: $ 10.08万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2895526
• 关键词: DNA directed RNA polymerase; DNA replication; RNA directed DNA polymerase; enzyme activity; frameshift mutation; genetic recombination; human immunodeficiency virus; microorganism culture; murine leukemia virus; nucleic acid sequence; nucleic acid structure; transfection /expression vector; virus DNA; virus RNA; virus genetics; virus replication

DESCRIPTION: Dr. Zhang proposes to compare the rate of mutation in vivo during first and second strand DNA synthesis. He will utilize an in vitro assay to compare the frameshift rates of RNA polymerase II, RT first strand, and RT second strand synthesis. He will test the three prevailing models for how recombination occurs in retroviruses using in vivo and in vitro systems. He will also test the effects of RNA secondary structure on stimulation of recombination.

描述：张博士提出比较体内突变率 在第一链和第二链 DNA 合成期间。 他将利用体外 比较 RNA 聚合酶 II、RT 第一链、 和RT第二链合成。 他将测试三种流行的模型 使用体内和体外实验了解逆转录病毒中重组如何发生 系统。 他还将测试RNA二级结构对 重组的刺激。