Novel chemoenzymatic synthesis and bioconjugation of peptides and proteins

肽和蛋白质的新型化学酶合成和生物共轭

• 批准号: LP170101085
• 负责人: Prof John Wade
• 金额: $ 44.14万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-06-21 至 2024-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP170101085
• 关键词: Novel; chemoenzymatic; synthesis; bioconjugation; peptides

This project aims to solve the critical difficulty of producing the complex peptide insulin and related insulin-like peptides (INSLs) and their analogues. The project will use unique, recyclable enzymes which ligate smaller peptide segments with extraordinary site and substrate selectivity. The expected outcome of the project is novel, cheaper and scalable, enzyme-mediated engineering of this critical class of peptides as biological probes and potential therapeutic agents.

本项目旨在解决复杂肽胰岛素及相关胰岛素样肽（INSLs）及其类似物生产的关键难题。该项目将使用独特的、可回收的酶，以非凡的位点和底物选择性连接较小的肽段。该项目的预期结果是新颖、廉价和可扩展的，酶介导的这类关键肽的工程作为生物探针和潜在的治疗剂。