CDC2-RELATED KINASES AND THE CONTROL OF CELL CYCLE

CDC2 相关激酶和细胞周期的控制

• 批准号: 2895987
• 负责人: EDWARD E HARLOW
• 金额: $ 30.08万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2895987
• 关键词: cell cycle proteins; cell growth regulation; cyclin dependent kinase; cyclins; enzyme activity; enzyme substrate; gene expression; immunochemistry; molecular cloning; monoclonal antibody; phosphorylation; polymerase chain reaction; protein purification; protein sequence; protein structure function; radiotracer; reagent /indicator

DESCRIPTION (adapted from investigator's abstract): During the last two years these investigators have learned several key properties of the cyclin-dependent kinases that have helped understand how these kinases recognize their substrates and how they might develop technical approaches to allow rapid identification of substrates for the cell-cycle-regulating kinases. The investigators have developed two techniques for screening of new substrates of the CDK2 kinases, enzymes that control the last steps of G1 and the early steps of DNA synthesis. They have identified six new potential cyclin E/CDK2 substrates to date. Two of the new substrates have been characterized in sufficient detail to believe that they are in vivo substrates. In this application, it is proposed to continue examination of how cyclin/CDK complexes recognize their substrates, to expand the validation of the substrate screening methods, to extend these screens to look for substrates for a limited set of other CDK family members, and to study the function of the new substrates through these screens.

描述（改编自研究者摘要）：在过去两年内 多年来，这些研究人员已经了解了几个关键的性质， 细胞周期蛋白依赖性激酶，帮助理解这些激酶 认识到它们的基质以及它们如何发展技术方法 为了快速鉴定细胞周期调节的底物， 激酶。 研究人员开发了两种技术来筛选 CDK 2激酶的新底物，控制最后步骤的酶， G1和DNA合成的早期步骤。 他们已经确定了六个新的 迄今为止潜在的细胞周期蛋白E/CDK 2底物。 其中两种新基质具有 已经足够详细地表征，以相信它们在体内 印刷受体. 在本申请中，建议继续审查 细胞周期蛋白/CDK复合物是如何识别其底物的， 验证底物筛选方法，将这些筛选扩展到 为有限的其他CDK家族成员寻找底物，并 通过这些屏幕研究新基质的功能。