MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM AVIUM

鸟分枝杆菌的分子流行病学

• 批准号: 2887307
• 负责人: Vivek Kapur
• 金额: $ 10.74万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887307
• 关键词: AIDS; Mycobacterium avium; electrophoresis; epidemiology; genetic markers; genetic strain; microorganism population study; molecular genetics; opportunistic infections; polymerase chain reaction; restriction fragment length polymorphism

DESCRIPTION (adapted from the Abstract): In the United States, infection with Mycobacterium avium is the most common disease of bacterial origin among patients with the acquired immunodeficiency syndrome (AIDS). Despite the great morbidity and mortality associated with M. avium infections, we lack a detailed understanding of the epidemiology of this pathogen. For instance, we do not yet know whether an animal reservoir exists of M. avium clones that cause disease in AIDS patients; we have much to learn about the transmission of the bacterium during the course of infection. In this application, the Principal Investigator proposes studies that will (a) provide a comprehensive understanding of the molecular epidemiology of M. avium infections in humans (primarily, in AIDS patients) and animals, and (b) enable the development of powerful new genetic markers for strain identification. Preliminary studies have led the Investigator to formulate three hypotheses: (i) the majority of M. avium infections in patients with AIDS are caused by only a small subset of extant M. avium clones; (ii) an animal reservoir exists of M. avium clones that may also be recovered from AIDS patients; and (iii) automated polymerase chain reaction (PCR)-based DNA fingerprinting strategies may be used for the rapid and unambiguous identification of M. avium clones. To test these hypotheses, the Investigator and his associates will use multilocus enzyme electrophoresis--the method of choice for bacterial population genetic studies--to determine allelic variation at metabolic enzyme loci in a sample of more that 1,000 M. avium isolates recovered from humans (primarily, from AIDS patients) and from domesticated and wild animals. All isolates in the collection will also be characterized by IS1245 fingerprinting, a technique that will provide a fine-structure epidemiologic analysis of M. avium from humans and animals. Finally, because present methodology for M. avium strain identification and fingerprinting are generally labor intensive and time consuming, the researchers will evaluate the feasibility of used a semi-automated PCR-based method (i.e., flourophore-enhanced repetitive-element PCR) for M. avium strain identification.

描述(摘自摘要)：在美国，感染 伴禽分枝杆菌是最常见的细菌性疾病 在患有获得性免疫缺陷综合征(AIDS)的患者中。尽管 与禽类分枝杆菌感染相关的巨大发病率和死亡率，我们 对这种病原体的流行病学缺乏详细的了解。为 例如，我们还不知道是否存在禽类分枝杆菌的动物宿主。 在艾滋病患者中引起疾病的克隆；我们有很多关于 细菌在感染过程中的传播。在这 申请，首席调查员提出的研究将：(A) 全面了解分支杆菌的分子流行病学。 人类(主要是艾滋病患者)和动物的禽类感染，以及 (B)为菌株开发强大的新遗传标记 身份证明。初步研究使调查员制定了 三个假设：(I)大多数禽类分枝杆菌感染患者 艾滋病只由现存的一小部分禽类分枝杆菌克隆引起； 存在禽类支原体克隆的动物储藏库，这些克隆也可以从 艾滋病患者；以及(Iii)基于自动聚合酶链式反应(PCR)的DNA 指纹识别策略可以用于快速和明确的 禽类根结线虫克隆的鉴定。为了检验这些假设， 调查员和他的同事将使用多位点酶 电泳法--细菌群体遗传研究的选择方法 研究--确定样本中代谢酶基因座的等位基因变异 在从人类身上发现的1,000多个禽类分支杆菌分离物中(主要来自 爱滋病患者)和家养和野生动物。所有的分离物都在 收藏品还将采用IS1245指纹识别技术 这将提供来自中国的禽类分支杆菌的精细结构流行病学分析。 人类和动物。最后，因为目前针对禽类分枝杆菌的方法学 菌株鉴定和指纹分析通常是劳动密集型的 由于耗时，研究人员将评估使用 基于半自动聚合酶链式反应的方法(即，荧光团增强的 重复元件聚合酶链式反应)用于禽类分支杆菌菌株的鉴定。