PHAGE DISPLAY GENERATED IMMUNOREAGENTS FOR HPV INFECTION

噬菌体展示产生的 HPV 感染免疫试剂

• 批准号: 2879182
• 负责人: GOAR GEVORKIAN
• 金额: $ 6.47万
• 依托单位: UNIVERSITY OF MEXICO, UNAM
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-06 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2879182
• 关键词: antiviral antibody; biomarker; cervix neoplasms; clinical research; diagnosis design /evaluation; enzyme linked immunosorbent assay; human papillomavirus; human subject; neoplasm /cancer diagnosis; peptide library; synthetic peptide; virus antigen

DESCRIPTION: (adapted from investigators abstract): Phage-display technology has emerged as a strategy to search for novel antigens and antibodies for diagnostics and vaccine development. It is known that the antibody repertoire in patients with HPV infection and cervical cancer is heterogeneous and the relevant antigens to be used for diagnosis cannot be identified in a number of cases. Using phage display peptide libraries it may be possible to identify epitopes that bind antibodies present in sera from patients with HPV infection and cervical cancer and which correspond to or mimic linear, discontinuous, or even non-peptidic epitopes of the cervical cancer-related agents. Also, phage display human IG single chain variable region fragments library will be used to isolate clones that specifically recognize the components in sera and cervical secretion samples from patients with cervical cancer and its precursors. These clones could then be used for diagnosis of HPV infection and or cervical cancer in phage-based ELISA. Synthetic peptides based on amino acid sequences of the selected epitopes will be prepared and used in peptide based ELISA.

描述：(改编自调查人员摘要)：噬菌体展示技术 已经成为一种寻找新的抗原和抗体的策略 诊断和疫苗开发。已知的抗体谱系 在HPV感染和宫颈癌患者中是异质性的 用于诊断的相关抗原不能在一些 案子。利用噬菌体展示多肽库，有可能识别 人乳头瘤病毒感染者血清中抗体的结合表位 和宫颈癌，并且对应于或模仿线性、不连续或 甚至是子宫颈癌相关因子的非肽表位。还有，噬菌体 展示人免疫球蛋白单链可变区片段文库将用于 分离特异性识别血清和宫颈中成分的克隆 宫颈癌患者及其先兆的分泌物样本。这些 克隆可用于HPV感染和/或宫颈癌的诊断。 在基于噬菌体的酶联免疫吸附试验中。基于天冬氨酸氨基酸序列的合成肽 选定的表位将被制备并用于基于多肽的酶联免疫吸附试验。