GLUCOCORTICOID RECEPTOR REGULATION BY PHOSPHATASE

磷酸酶对糖皮质激素受体的调节

• 批准号: 2906414
• 负责人: MICHAEL DAVID CHINKERS
• 金额: $ 19.36万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2906414
• 关键词: biological signal transduction; corticosteroid receptors; fluorescence microscopy; genetic transcription; glucocorticoids; green fluorescent proteins; immunoprecipitation; intracellular transport; laboratory rabbit; molecular chaperones; mutant; phosphoprotein phosphatase; phosphorylation; protein folding; receptor binding; receptor coupling; receptor expression; stress proteins; tissue /cell culture; transfection

DESCRIPTION (adapted from applicant's abstract): The objective of this application is to understand the role of protein phosphatase 5 (PP5) in GR signaling as a molecular chaperone and in regulating signal transduction by steroid receptors and other molecules. Specifically, Aim 1 will define at what point of the GR signaling pathway PP5 is required; Aim 2 will test whether PP5 regulates the phosphorylation state of the GR and other proteins in the GR heterocomplex; Aim 3 will test whether PP5 has a general function as a molecular chaperone of other proteins associated with hsp90.

描述（改编自申请人的摘要）：此目的 应用是了解蛋白质磷酸酶5（PP5）在 GR信号作为分子伴侣和调节信号 类固醇受体和其他分子转导。 具体来说， AIM 1将定义GR信号通路PP5的位置 必需的; AIM 2将测试PP5是否调节磷酸化 GR异质复合体中GR和其他蛋白质的状态；目标3意志 测试PP5是否具有一般功能作为分子伴侣的一般函数 其他与HSP90相关的蛋白质。