EFFECT OF GROWTH HORMONE ON CRANIOFACIAL PROPORTIONS

生长激素对颅面比例的影响

• 批准号: 3072216
• 负责人: BRIAN SHEA
• 金额: $ 6.4万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3072216
• 关键词: Ceboidea; bite strength; cephalometry; dental occlusion; dentinogenesis; endocrine disorder; face; genetically modified animals; injection /infusion; laboratory mouse; malocclusion; model design /development; physiologic bone resorption; scanning electron microscopy; skull; somatotropin

This research investigates the effects of variation in growth hormone (GH) on facial growth,dental size and development, and occlusion. Craniofacial proportion changes, localized patterns of bone deposition and resorption, and tooth size, shape, development and occlusion will be examined in three primary models of endocrine growth disturbance: 1) giant transgenic (MTrGH) mice; 2) Snell dwarf (dw/dw) mutant mice; and 3) GH-administered marmoset monkeys. Generally stated, the hypothesis to be tested in this work is that in all three experimental groups changes in the size and shape of the craniofacial complex will result from differential extension (in the transgenic mice, marmosets) or truncation (in the dwarf mice) of the patterns of allometric (relative) growth observed in the respective control groups. In addition, differences in craniofacial proportions between experimentals and controls are hypothesized to result from differences in general rates of bone resorption and deposition, as opposed to fundamental shifts in these basic regional patterns themselves, and the size, shape and patterns of eruption and occlusion of the teeth are hypothesized to be similar in both experimental and control groups. These hypotheses will be tested by utilizing linear dimensions and coordinate points taken from skulls and radiographs. Scanning electron microscopic examination of bone surfaces will determine the distribution of patterns of resorption and deposition during growth. My career objectives are to continue my preliminary studies of the influence of growth hormones on patterns of skeletal growth and proportions, and to extend these more fully into the experimental and clinical realms. An RCDA would particularly advance my research career by dramatically reducing my unusually heavy teaching load which is an obstacle to the effective implementation of involved and prolonged laboratory and clinical investigations. Moreover, this award would allow me to develop new research directions in my investigations of skeletal growth and proportions, particularly longitudinal and experimental studies of growth hormone administration in a primate model, and the use of SEM analysis in the determination of patterns of bone deposition and resorption in the craniofacial complex during normal and abnormal growth. The Institution's support plans if an RCDA is awarded include a reduction of my normal teaching load by more than half, such that total time allotted for the research described would be 80% with no more than 20% of my time spent on related teaching and advising activities. In addition, access to specific research resources including equipment as well as training in growth hormone (GH) administration experiments and the determination of growth hormone levels will be provided by my home departments and others here at Northwestern.

本研究探讨了生长激素（GH）变化的影响， 面部生长、牙齿大小和发育以及咬合。颅面 比例变化，骨沉积和吸收的局部模式， 牙齿的大小、形状、发育和咬合将在三个 内分泌生长障碍的主要模型：1）巨大转基因（MTrGH） 小鼠; 2）Snell侏儒（dw/dw）突变小鼠;和3）GH给药的绒猴 猴子一般来说，在这项工作中要检验的假设是， 在所有三个实验组中， 颅面复合体将由差异延伸（在 转基因小鼠，绒猴）或截短（侏儒小鼠） 在相应对照中观察到的异速生长（相对）模式 组此外，颅面比例的差异， 假设实验和对照是由以下因素的差异造成的： 骨吸收和沉积的一般速率，而不是基本的 这些基本区域格局本身的变化，以及这些区域的规模、形状和 牙齿的萌出和咬合模式被假设为 在实验组和对照组中相似。这些假设将是 通过使用线性尺寸和坐标点进行测试， 头骨和X光片骨扫描电镜检查 表面将决定再吸收模式的分布， 生长过程中的沉淀。 我的职业目标是继续我的初步研究 生长激素对骨骼生长模式的影响 比例，并将其更充分地扩展到实验和 临床领域一个RCDA将特别促进我的研究生涯， 极大地减轻了我异常繁重的教学负担， 有效实施涉及的和长期的实验室和 临床研究。此外，这个奖项将使我能够开发新的 在我的骨骼生长研究方向， 比例，特别是增长的纵向和实验研究 在灵长类动物模型中的激素施用，以及SEM分析在 骨沉积和骨吸收模式的测定 颅面复合体在正常和异常生长。 该机构的支持计划，如果一个RCDA被授予包括减少 我的正常教学量的一半以上，这样，总的时间分配 因为我所描述的研究将是80%，而我的时间不超过20 用于相关的教学和咨询活动。此外，获得 具体的研究资源，包括设备和培训， 生长激素（GH）给药实验和测定 生长激素水平将由我的家庭部门和其他人提供 在西北大学