AFFINITY LABELING OF THE ADIOPCYTE HEXOSE CARRIER
AFFINITY LABELING OF THE ADIOPCYTE HEXOSE CARRIER
批准号:
3072516
负责人:
JAMES M. MAY
金额:
$5.1万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-09-01 至 1988-06-30
中文摘要
重点将放在permeans和impermeans的用法上
英文摘要
The major emphasis will be on the use of both permeant and impermeant
affinity labels for the hexose carrier in rat adipocytes and human
erythrocytes. In the previous years several potentially useful derivatives
were developed, all of which use a sugar as the affinity ligand. The
initial testing of these derivatives will be completed by demonstrating
that they either are permeant or impermeant to cells, that they inhibit
transport irreversibly upon binding to the carrier site, that their action
is inhibited by D-glucose and other substrate analogues, and that they bind
the carrier as a linear function of transport inhibition. The major
derivatives to be tested will be a cell-permeant carbon-6 modified glucose
derivative having an aryl azide as the coupling agent, and an impermeant
bis(mannose) derivative having either an aryl azide or maleimide coupling
group. The ability of these derivatives to label the hexose carrier either
in the plasma membrane or in the case of adipocytes in intracellular
organelles will then be tested. In adipocytes particular attention will be
paid to the effects of insulin, which are to translocate intracellular
carriers to the plasma membrane. It will also be important to identify the
hexose carrier under various conditions. Carrier identity using one or
more of these labels will be confirmed with the use of either a monoclonal
or polyclonal antibody made to the erythrocyte hexose carrier. When it has
been established that these derivatives can label either externally
disposed carrier in whole cells or internal carriers, the kinetics of the
translocation process will be analyzed in detail. Particular emphasis will
be paid to the possibility that carriers recycle to and from the cell
surface, and that this recycling phenomenon may be part of a more general
process of membrane protein turnover in adipocytes. Previous studies based
on the finding that hexose transport in erythrocytes may be regulated by
ATP depletion will also be extended. These studies will explore the
possibility that the carrier or a regulatory protein is phosphorylated and
thus activated in these cells. Such a mechanism may provide an alternate
means for activating hexose transport. The major significance of this work
will be to demonstrate that a functional membrane protein can be affinity
labeled in erythrocytes and in insulin sensitive adipocytes and that such
affinity labeling can be used to monitor the disposition of membrane
proteins under various conditions of stimulation or inhibition.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Failure of the glutamate uptake-ascorbic acid exchange drives seizure susceptibility and severity
-
批准号:9892973
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:JAMES M. MAY
-
依托单位:
Failure of the glutamate uptake-ascorbic acid exchange drives seizure susceptibility and severity
-
批准号:9352655
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:JAMES M. MAY
-
依托单位:
Failure of the glutamate uptake-ascorbic acid exchange drives seizure susceptibility and severity
-
批准号:10683056
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:JAMES M. MAY
-
依托单位:
Failure of the glutamate uptake-ascorbic acid exchange drives seizure susceptibility and severity
-
批准号:10179345
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:JAMES M. MAY
-
依托单位:
Ascorbic Acid Function and Metabolism
-
批准号:7899389
-
项目类别:
-
资助金额:$10.0万
-
财政年份:2009
-
负责人:JAMES M. MAY
-
依托单位:
Vitamin C Transporters in the Brain
-
批准号:8096657
-
项目类别:
-
资助金额:$29.62万
-
财政年份:2008
-
负责人:JAMES M. MAY
-
依托单位:
Vitamin C Transporters in the Brain
-
批准号:7526762
-
项目类别:
-
资助金额:$30.22万
-
财政年份:2008
-
负责人:JAMES M. MAY
-
依托单位:
Vitamin C Transporters in the Brain
-
批准号:7624264
-
项目类别:
-
资助金额:$30.22万
-
财政年份:2008
-
负责人:JAMES M. MAY
-
依托单位:
Vitamin C Transporters in the Brain
-
批准号:7860690
-
项目类别:
-
资助金额:$29.92万
-
财政年份:2008
-
负责人:JAMES M. MAY
-
依托单位:
ANITOXIDANT FUNCTIONS OF LIPOIC ACID
-
批准号:7605547
-
项目类别:
-
资助金额:$0.55万
-
财政年份:2006
-
负责人:JAMES M. MAY
-
依托单位:
ANITOXIDANT FUNCTIONS OF LIPOIC ACID
-
批准号:7731372
-
项目类别:
-
资助金额:$0.03万
-
财政年份:2006
-
负责人:JAMES M. MAY
-
依托单位:
ANITOXIDANT FUNCTIONS OF LIPOIC ACID
-
批准号:7375606
-
项目类别:
-
资助金额:$1.22万
-
财政年份:2005
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant vitamins in models of Alzheimer's Disease
-
批准号:6989030
-
项目类别:
-
资助金额:$31.52万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant vitamins in models of Alzheimer's Disease
-
批准号:7365159
-
项目类别:
-
资助金额:$29.99万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant vitamins in models of Alzheimer's Disease
-
批准号:6718831
-
项目类别:
-
资助金额:$32.28万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant vitamins in models of Alzheimer's Disease
-
批准号:7173812
-
项目类别:
-
资助金额:$30.6万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant vitamins in models of Alzheimer's Disease
-
批准号:6843098
-
项目类别:
-
资助金额:$32.28万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
ANITOXIDANT FUNCTIONS OF LIPOIC ACID
-
批准号:7207242
-
项目类别:
-
资助金额:$0.09万
-
财政年份:2004
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant Functions of Lipoic Acid
-
批准号:6685423
-
项目类别:
-
资助金额:$18.88万
-
财政年份:2003
-
负责人:JAMES M. MAY
-
依托单位:
Antioxidant Functions of Lipoic Acid
-
批准号:6772529
-
项目类别:
-
资助金额:$18.88万
-
财政年份:2003
-
负责人:JAMES M. MAY
-
依托单位:
国内基金
海外基金
支链氨基酸代谢紊乱调控“Adipocytes - Macrophages Crosstalk”诱发2型糖尿病脂肪组织功能和结构障碍的作用及机制
-
批准号:81970721
-
项目类别:面上项目
-
资助金额:55.0万元
-
批准年份:2019
-
负责人:陶凌
-
依托单位: