BIOCHEMISTRY OF GENETIC RECOMBINATION

基因重组的生物化学

• 批准号: 3070705
• 负责人: Michael M. Cox
• 金额: $ 4.69万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3070705
• 关键词: Escherichia coli; adenosine triphosphate; bacterial DNA; bacterial genetics; bacteriophage M13; bacteriophage phi X174; circular DNA; electron microscopy; gel electrophoresis; gene conversion; genetic crossing over; genetic manipulation; genetic recombination; plasmids; radioimmunoassay; yeasts

Two type of genetic recombination events will be studied using defined in vitro systems and purified proteins derived from bacteria and yeast. The proteins to be studied are: 1) The FLP protein of the yeast 2 micron plasmid. This protein promotes a site-specific recombination event which results in the inversion of a segment of the 2 micron plasmid DNA relative to the rest of the plasmid. 2) The recA protein of E. coli. This protein plays a central role in homologous or general genetic recombination in bacteria. The long range goal of this project is to elucidate the chemical mechanisms by which these and similar proteins promote genetic recombination. The work will employ the full range of chemical, kinetic, physical, and genetic techniques available for biochemical analysis. Benefits to be derived from this work include an improved understanding of recombination events in eukaryotes, including those involved in the generation of antibody diversity and in the transformation of mammalian cells by a number of DNA tumor viruses, and the development of new technologies in recombinant DNA research.

两种类型的遗传重组事件将研究使用定义在 体外系统和来自细菌和酵母的纯化蛋白质。 的 要研究的蛋白质是： 1)酵母2微米质粒的FLP蛋白。 这种蛋白质促进 位点特异性重组事件，其导致 相对于质粒的其余部分，2微米质粒DNA的片段。 2)重组E.杆菌 这种蛋白质在 细菌中的同源或一般遗传重组。 这个项目的长期目标是阐明化学机制 这些和类似的蛋白质通过其促进基因重组。 的 工作将采用全方位的化学，动力学，物理，和遗传 可用于生化分析的技术。 从这项工作中获得的好处包括更好地了解 真核生物中的重组事件，包括那些参与 抗体多样性的产生和哺乳动物细胞的转化 细胞的DNA肿瘤病毒的数量，并开发新的 重组DNA研究技术。