IRON ACQUISITION BY STAPHYLOCOCCI

血清前转铁蛋白可抑制葡萄球菌的生长。 铁结合蛋白。据推测，在人类感染期间的增长也是 依赖于葡萄球菌从宿主获取铁的能力。 尽管之前的研究已经检查了 革兰氏阴性生物获得铁，几乎没有做过什么工作来 阐明革兰氏阳性细菌使用的机制。几十年来， 金黄色葡萄球菌一直是社区的重要事业--和 医院获得性血液感染。最近的葡萄球菌 表皮萎缩症已成为医院感染的最常见原因之一。 血液感染。然而，人们对这一机制知之甚少。 葡萄球菌与载脂蛋白竞争。 该提案的长期目标是制定一项全面的 金黄色葡萄球菌获取铁的机制图。化验分析 (55)包括载脂蛋白在内的各种底物对铁的吸收将是 用来确定哪一种作为铁源。其他人使用的机制 将对细菌进行评估，以确定它们是否在铁中起作用 葡萄球菌感染：细菌铁络合剂(铁载体)， 细菌还原酶，细菌或宿主衍生的有机酸。机制 将与金黄色葡萄球菌使用的 毒力较弱的葡萄球菌。 临床研究人员奖将使首席研究人员(PI) 解决有关葡萄球菌发病机制的关键问题 感染。这些数据将为了解这些差异提供重要的见解 金黄色葡萄球菌和毒性较弱的葡萄球菌引起的感染之间的关系 物种。其目标是开发铁的抗菌活性。 将剥夺纳入预防和治疗糖尿病的新途径 葡萄球菌感染。此外，私家侦探还将获得经验 对她未来作为独立调查员的职业生涯至关重要。

The growth of staphylococci can be inhibited by aportransferrin, the serum iron-binding protein. Presumably, growth during human infections is also dependent on the ability of staphylococci to acquire iron from the host. Although previous research has examined the mechanisms by which gram-negative organisms acquire iron, little work has been done to elucidate the mechanisms used by gram-positive organisms. For decades, Staphylococcus aureus has been an important cause of community- and hospital-acquired bloodstream infections. Recently Staphylococcus epidermidis has become one of the most common causes of nosocomial bloodstream infections. Yet little is known about the mechanisms by which staphylococci compete with apotransferrin. The long-term objective of the proposal is to develop a comprehensive picture of the mechanisms by which staphylococci acquire iron. Assays of (55)Fe uptake from various substrates, including apotransferrin, will be used to determine which serve as iron sources. Mechanisms used by other bacteria will be evaluated to determine whether they have a role in iron acquisition by staphylococci: bacterial iron chelators (siderophores), bacterial reductases, bacteria- or host-derived organic acids. Mechanisms used by S. aureus will be compared and contrasted with those used by the less virulent staphylococcal species. The Clinical Investigator Award will enable the Principal Investigator (PI) to address critical questions regarding the pathogenesis of staphylococcal infections. The data will give important insights into the differences between infections caused by S. aureus and by less virulent staphylococcal species. The goal is to develop the antibacterial activity of iron deprivation into new approaches for the prevention and treatment of staphylococcal infections. In addition, the PI will gain experience essential for her future career as an independent investigator.