MONOCLONAL ANTIBODIES TO FETAL EPIDERMIS

人胎儿皮肤的免疫荧光鉴定技术分析 层粘连蛋白、大疱性类天疱疮抗原、 用单抗鉴定天疱疮抗原和锚定原纤维 抗体AF-1和AF-2。产生了额外的单抗。 来自用人胎儿皮肤免疫的小鼠。特异性单抗 产生的与人表皮细胞角蛋白、人胎儿 周皮和基膜带。改进的分离技术 取自真皮的人胎儿表皮使小鼠能够免疫 更早的人类胎儿组织，以努力开发单克隆抗体 到非常早期的发育抗原。 在未来，目前开发的单抗的具体特征 抗体将需要更密集的生化分析，有两个 空间凝胶技术和免疫沉淀。新技术的发展 用人胎儿免疫的小鼠将产生单抗 取胎龄12周前的表皮组织。进一步 体内、体外(裸鼠移植)的研究进展 胎儿角质形成细胞培养系统可能允许产生的单克隆化 抗体在胎儿发育过程中作为分子控制和反应的探针 发展。此外，这些单抗将被用于 良恶性表皮鉴别的继续评价 肿瘤和遗传性疾病。

Analysis of human fetal skin by immunofluorescent techniques identified sequential antigen development of laminin, bullous pemphigoid antigen, pemphigus antigen and anchoring fibril antigen identified by monoclonal antibodies AF-1 and AF-2. Additional monoclonal antibodies were generated from mice immunized with human fetal skin. Specific monoclonal antibodies generated reacted with cytokeratins of human epidermis, human fetal periderm and basement membrane zone. Improved techniques of separating human fetal epidermis from dermis have allowed mice to be immunized with earlier human fetal tissues in an effort to develop monoclonal antibodies to very early antigens of development. In the future, specific characterization of currently developed monoclonal antibodies will require more intense biochemical analysis with two dimensional gel techniques and immunoprecipitation. Development of new monoclonal antibodies will result from mice immunized with human fetal epidermal tissues obtained before 12 weeks gestational age. Further development of current in vitro and in vivo (transplanted to nude mice) fetal keratinocyte culture systems may allow the generated monoclonal antibodies to act as probes of molecular control and response during fetal development. In addition these monoclonal antibodies will be used to continue evaluation of differentiating benign and malignant epidermal neoplasms and genetic disorders.