MONOCLONAL ANTIBODIES TO FETAL EPIDERMIS

胎儿表皮单克隆抗体

• 批准号: 3079130
• 负责人: ALFRED T LANE
• 金额: $ 7.08万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3079130
• 关键词: basal cell carcinoma; basement membrane; cell bank /registry; embryo /fetus culture; epidermolysis bullosa; gel electrophoresis; human tissue; hypoxanthines; immunofluorescence technique; keratoacanthoma; keratosis; membrane activity; methotrexate; monoclonal antibody; multiple myeloma; orphan disease /drug; prenatal growth disorder; preneoplastic state; psoriasis; radioimmunoassay; skin; skin neoplasms; squamous cell carcinoma; surface antigens; thymidine; tissue /cell culture; warts

Analysis of human fetal skin by immunofluorescent techniques identified sequential antigen development of laminin, bullous pemphigoid antigen, pemphigus antigen and anchoring fibril antigen identified by monoclonal antibodies AF-1 and AF-2. Additional monoclonal antibodies were generated from mice immunized with human fetal skin. Specific monoclonal antibodies generated reacted with cytokeratins of human epidermis, human fetal periderm and basement membrane zone. Improved techniques of separating human fetal epidermis from dermis have allowed mice to be immunized with earlier human fetal tissues in an effort to develop monoclonal antibodies to very early antigens of development. In the future, specific characterization of currently developed monoclonal antibodies will require more intense biochemical analysis with two dimensional gel techniques and immunoprecipitation. Development of new monoclonal antibodies will result from mice immunized with human fetal epidermal tissues obtained before 12 weeks gestational age. Further development of current in vitro and in vivo (transplanted to nude mice) fetal keratinocyte culture systems may allow the generated monoclonal antibodies to act as probes of molecular control and response during fetal development. In addition these monoclonal antibodies will be used to continue evaluation of differentiating benign and malignant epidermal neoplasms and genetic disorders.

通过免疫荧光技术分析人类胎儿皮肤 层粘连蛋白、大疱性类天疱疮抗原的连续抗原形成， 单克隆鉴定天疱疮抗原和锚定原纤维抗原 抗体 AF-1 和 AF-2。 生成了额外的单克隆抗体 来自用人类胎儿皮肤免疫的小鼠。 特异性单克隆抗体 与人表皮、人胎儿的细胞角蛋白反应生成 周皮和基底膜区。 分离技术的改进 来自真皮的人类胎儿表皮已允许小鼠免疫 早期人类胎儿组织以开发单克隆抗体 发育的早期抗原。 未来，目前开发的单克隆抗体的具体表征 抗体将需要更深入的生化分析，包括两种 三维凝胶技术和免疫沉淀。 开发新 单克隆抗体将由用人胎儿免疫的小鼠产生 12周胎龄前获得的表皮组织。 更远 目前的体外和体内发育（移植到裸鼠） 胎儿角质形成细胞培养系统可能会产生单克隆 抗体作为胎儿时期分子控制和反应的探针 发展。 此外，这些单克隆抗体将用于 继续评估区分良性和恶性表皮 肿瘤和遗传性疾病。