MONOCLONAL ANTIBODIES TO FETAL EPIDERMIS

胎儿表皮单克隆抗体

• 批准号: 3079129
• 负责人: ALFRED T LANE
• 金额: $ 7.08万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3079129
• 关键词: basal cell carcinoma; basement membrane; cell bank /registry; embryo /fetus culture; epidermolysis bullosa; gel electrophoresis; human tissue; hypoxanthines; immunofluorescence technique; keratoacanthoma; keratosis; membrane activity; methotrexate; monoclonal antibody; multiple myeloma; orphan disease /drug; prenatal growth disorder; preneoplastic state; psoriasis; radioimmunoassay; skin; skin neoplasms; squamous cell carcinoma; surface antigens; thymidine; tissue /cell culture; warts

Analysis of human fetal skin by immunofluorescent techniques identified sequential antigen development of laminin, bullous pemphigoid antigen, pemphigus antigen and anchoring fibril antigen identified by monoclonal antibodies AF-1 and AF-2. Additional monoclonal antibodies were generated from mice immunized with human fetal skin. Specific monoclonal antibodies generated reacted with cytokeratins of human epidermis, human fetal periderm and basement membrane zone. Improved techniques of separating human fetal epidermis from dermis have allowed mice to be immunized with earlier human fetal tissues in an effort to develop monoclonal antibodies to very early antigens of development. In the future, specific characterization of currently developed monoclonal antibodies will require more intense biochemical analysis with two dimensional gel techniques and immunoprecipitation. Development of new monoclonal antibodies will result from mice immunized with human fetal epidermal tissues obtained before 12 weeks gestational age. Further development of current in vitro and in vivo (transplanted to nude mice) fetal keratinocyte culture systems may allow the generated monoclonal antibodies to act as probes of molecular control and response during fetal development. In addition these monoclonal antibodies will be used to continue evaluation of differentiating benign and malignant epidermal neoplasms and genetic disorders.

用免疫荧光技术对人胎儿皮肤进行分析鉴定