MAPPING AND ISOLATION OF THE DB GENE
DB 基因的定位和分离
基本信息
- 批准号:3080715
- 负责人:
- 金额:$ 7.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-07-01 至 1993-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Despite strong evidence that the tendency towards obesity in
humans is inherited the genes contributing to this neuroendocrine
predisposition are not known. It is the goal of this project to
construct a large scale physical map of and ultimately isolate the
single gene that is mutated in a classical mouse model of obesity,
the db/db mouse. This is an extensive proposed project in which
serendipity may play an important role. Nonetheless, several
systematic and complementary approaches can be utilized to
make a directed attempt at identifying this important gene.
Two vector systems will be employed to clone mouse chromosome
4 DNA fragments derived from a mouse/hamster hybrid cell line.
A cosmid library will be constructed to isolate DNA fragments
20-50 kilobases in size and a yeast artificial chromosome library
employing a system recently developed at Washington University
will be constructed to isolate DNA fragments 100-700 kilobases in
size. From these contiguous clones a detailed large scale genetic
map of the region surrounding the db mutation will be assembled
utilizing pulsed field gel electrophoresis, indirect end-label
mapping, and hybridization with a panel of random oligonucleotide
probes. Subsequently, a systematic screening of the derived gene
sequences for the db locus wll be undertaken. Three approaches
will be used: 1) searching for small deletions in this cloned region
of mouse chromosome 4, 2) looking for variant or absent mRNA
sequences in tissues such as brain (specifically hypothalamus)
which are possible sites of expression of an abnormal db encoded
gene product, and 3) screening sequences from this cloned region
for clusters of non-methylated CpG dinucleotides which have been
found to occur at the sites of expressed genes.
尽管有强有力的证据表明肥胖的趋势
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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FREDERICK T FIEDOREK其他文献
FREDERICK T FIEDOREK的其他文献
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{{ truncateString('FREDERICK T FIEDOREK', 18)}}的其他基金
MAPPING AND STRUCTURAL ANALYSIS OF MOUSE OBESITY GENES
小鼠肥胖基因的定位和结构分析
- 批准号:
3245560 - 财政年份:1991
- 资助金额:
$ 7.11万 - 项目类别:
MAPPING AND STRUCTURAL ANALYSIS OF MOUSE OBESITY GENES
小鼠肥胖基因的定位和结构分析
- 批准号:
2143495 - 财政年份:1991
- 资助金额:
$ 7.11万 - 项目类别:
MAPPING AND STRUCTURAL ANALYSIS OF MOUSE OBESITY GENES
小鼠肥胖基因的定位和结构分析
- 批准号:
3245561 - 财政年份:1991
- 资助金额:
$ 7.11万 - 项目类别:
MAPPING AND STRUCTURAL ANALYSIS OF MOUSE OBESITY GENES
小鼠肥胖基因的定位和结构分析
- 批准号:
3245559 - 财政年份:1991
- 资助金额:
$ 7.11万 - 项目类别:
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