STRETCH AS A GROWTH STIMULUS IN VASCULAR SMOOTH MUSCLE

平滑肌异常生长在肺血管中的重要作用 疾病，目前尚不清楚是什么调节了它，但临床上 实验观察表明，机械刺激可能会 发挥重要作用。我建议检验一下这一假设 拉伸对肺组织生长和蛋白质合成的调节作用 血管平滑肌细胞。我会用培养的细胞 确定拉伸是否会刺激增生症或 肥大。拉伸对整体蛋白质合成的影响， 以及特定的蛋白质将被确定。如果，如 预料到，拉伸确实有生理作用(S)，其 机制将被研究，细胞内的“信号”与 增长是最初调查的重点。终极目标 就是在分子水平上了解机械刺激如何影响 平滑的肌肉细胞。 这个项目应该会给我提供一个很好的分子基础。 生物学：它需要在几个基本方面集中精力 分子生物学的，需要集中在几个 分子生物学的基本方面和在许多方面的经验 重要的实验技术。纳达尔-吉纳德博士的经历 清晰和批判性的思维对我的帮助应该是无价的 在科学上成熟。最后，在实验室里 拟开展的项目设备齐全，非常 活跃，有一大批调查人员对许多 分子和细胞生物学方面。

Abnormal growth of smooth muscle is important in pulmonary vascular disease, and it is not known what modulates it, but both clinical and experimental observations suggest that mechanical stimuli may play an important role. I propose to examine the hypothesis that stretch acts to modulate growth and protein synthesis in pulmonary vascular smooth muscle cells. I will use cultured cells to determine whether stretch stimulates either hyperplasia or hypertrophy. The effect of stretch on overall protein synthesis, as well as on specific protein will be determined. If, as anticipated, stretch does have a physiological effect(s), its mechanisms will be studied, with intracellular "signal" linked with growth being the initial focus of investigation. The ultimate goal is to understand on a molecular level how mechanical stimuli affect smooth muscle cells. This project should afford me an excellent grounding in molecular biology: It requires concentration in several fundamental aspects of molecular biology and requires concentration in several fundamental aspects of molecular biology and experience in many important experimental techniques. Dr. Nadal-Ginard's experience and clear and critical thinking should be invaluable in helping me mature scientifically. Finally, the laboratory in which the proposed project will be undertaken is well equipped and very active, with a "critical mass" of investigators interested in many aspects of molecular and cellular biology.