STRETCH AS A GROWTH STIMULUS IN VASCULAR SMOOTH MUSCLE

几种疾病会引起肺部的病理变化。 血管床。这种“肺血管病”可能会导致右 心力衰竭和过早死亡。细胞事件 这些病理变化背后的原因人们知之甚少，但它 明确了血管的异常生长和合成功能 平滑的肌肉很重要。虽然还不知道是什么 调节这种异常的平滑肌功能，临床和 实验观察表明，机械刺激可能起作用。 一个重要的角色。 有人建议检验这样一种假设，即拉伸作为一种 肺血管平滑肌生长刺激剂。先前 大鼠肺血管平滑肌细胞的鉴定和培养 动脉将生长在胶原蛋白涂层的硅胶膜上， 进行阶梯式或重复拉伸以确定：1)是否 拉伸影响有丝分裂的比率(通过~3H-胸腺嘧啶核苷)、细胞大小、 和蛋白质含量。生长相关原基因的表达- 癌基因c-myc和c-fos(通过Northern杂交分析)也将是 下定决心。2.如果拉伸调节整体蛋白质生产 (由~3H-亮氨酸和35S-蛋氨酸)，以及这是否受到影响 通过改变蛋白质合成的速度(总和 核糖体RNA、氨基酸摄取和S6激酶激活)和/或 降解(使用~3H-苯丙氨酸)。3)如果拉伸进行调制 收缩蛋白(肌球蛋白重链和调节轻链， 平滑肌特异的α-肌动蛋白和平滑肌特异的 α-原肌球蛋白)消息或蛋白质产生，和/或胶原蛋白 弹性蛋白(凝胶电泳法和免疫沉淀法) 综合。4)如果如预期的那样，Stretch确实具有 生理效应(S)，其机制将被研究，与 与生长有关的细胞内“信号”(钠内流， 细胞内pH和(Ca)，以及Na/K ATP酶的激活) 是最初调查的重点。 拟议研究的最初目标是确定哪些 伸展运动可能会影响平滑肌功能， 探索拉伸影响细胞生理的机制(S)。 从长远来看，人们希望探测器可以发展成 使在体内探索机械的作用成为可能 血管病理学中的变形。

Several disorders cause pathological changes in the pulmonary vascular bed. This "pulmonary vascular disease" may lead to right ventricular failure and premature death. The cellular events underlying these pathologic changes are poorly understood, but it is clear that abnormal growth and synthetic function of vascular smooth muscle are important. Although it is not known what modulates this abnormal smooth muscle function, clinical and experimental observations suggest that mechanical stimuli may play an important role. It is proposed to examine the hypothesis that stretch acts as a growth stimulus for pulmonary vascular smooth muscle. Previously characterized, cultured smooth muscle cells from rat pulmonary artery will be grown on a collagen-coated silicone membrane and subjected to step or repetitive stretch to determine: 1) Whether stretch affects the rate of mitosis (by 3H-thymidine), cell size, and protein content. Expression of the growth-associated proto- oncogenes c-myc and c-fos (by Northern blot analysis) will also be determined. 2. If stretch modulates overall protein production (by 3H-leucine and 35S-methionine), and whether this is effected by an altered rate of protein synthesis (measurement of total and ribosomal RNA, amino acid uptake, and S6 kinase activation) and/or degradation (using 3H-phenylalanine). 3) If stretch modulates contractile protein (myosin heavy and regulatory light chains, smooth muscle specific alpha actin, and smooth muscle specific alpha-tropomyosin) message or protein production, and/or collagen and elastin (gel electrophoresis and immunoprecipitation) synthesis. 4) If, as anticipated, stretch does have a physiological effect(s), its mechanism will be studied, with intracellular "signals" linked with growth (Na+ influx, intracellular pH and (Ca++), and activation of Na+/K+ ATP-ase) being the initial focus of investigation. The initial goal of the proposed studies is to determine which smooth muscle functions may be influenced by stretch, and to explore the mechanism(s) by which stretch affects cell physiology. Over the longer run, it is hoped that probes may be developed to make possible in vivo explorations of the role of mechanical deformation in vascular pathology.