STRETCH AS A GROWTH STIMULUS IN VASCULAR SMOOTH MUSCLE

拉伸作为血管平滑肌的生长刺激

• 批准号: 3082612
• 负责人: THOMAS JONATHAN KULIK
• 金额: $ 6.9万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3082612
• 关键词: autoradiography; blood vessel disorder; collagen; complementary DNA; densitometry; elastin; fluorescence microscopy; gel electrophoresis; gene expression; growth /development; hyperplasia; hypertrophy; immunofluorescence technique; laboratory rat; mechanical stress; membrane transport proteins; molecular biology; muscle proteins; nucleic acid hybridization; nucleic acid probes; phase contrast microscopy; protein degradation; protooncogene; pulmonary artery; pulmonary circulation; pulmonary veins; scintillation counter; tissue /cell culture; vascular smooth muscle

Abnormal growth of smooth muscle is important in pulmonary vascular disease, and it is not known what modulates it, but both clinical and experimental observations suggest that mechanical stimuli may play an important role. I propose to examine the hypothesis that stretch acts to modulate growth and protein synthesis in pulmonary vascular smooth muscle cells. I will use cultured cells to determine whether stretch stimulates either hyperplasia or hypertrophy. The effect of stretch on overall protein synthesis, as well as on specific protein will be determined. If, as anticipated, stretch does have a physiological effect(s), its mechanisms will be studied, with intracellular "signal" linked with growth being the initial focus of investigation. The ultimate goal is to understand on a molecular level how mechanical stimuli affect smooth muscle cells. This project should afford me an excellent grounding in molecular biology: It requires concentration in several fundamental aspects of molecular biology and requires concentration in several fundamental aspects of molecular biology and experience in many important experimental techniques. Dr. Nadal-Ginard's experience and clear and critical thinking should be invaluable in helping me mature scientifically. Finally, the laboratory in which the proposed project will be undertaken is well equipped and very active, with a "critical mass" of investigators interested in many aspects of molecular and cellular biology.

平滑肌的异常生长对肺血管很重要 疾病，尚不清楚是什么调节它，但临床上 实验观察表明机械刺激可能 发挥重要作用。 我建议检验以下假设 拉伸作用调节肺的生长和蛋白质合成 血管平滑肌细胞。 我将使用培养细胞 确定拉伸是否会刺激增生或 肥大。 拉伸对整体蛋白质合成的影响， 以及特定蛋白质也将被确定。 如果，如 预期，拉伸确实具有生理效应，其 将研究与细胞内“信号”相关的机制 增长是最初调查的重点。 最终目标 是在分子水平上理解机械刺激如何影响 平滑肌细胞。 这个项目应该为我提供良好的分子基础 生物学：需要专注于几个基本方面 分子生物学，需要专注于几个 分子生物学的基本方面和许多方面的经验 重要的实验技术。 纳达尔-吉纳德博士的经历 清晰而批判性的思维对我的帮助是无价的 科学上成熟。 最后，实验室 拟议的项目将进行装备精良，非常 活跃，拥有对许多领域感兴趣的“临界数量”的调查人员 分子和细胞生物学的各个方面。