INSULIN-SPECIFIC ACTIVATION OF IMMUNE T CELLS

免疫 T 细胞的胰岛素特异性激活

• 批准号: 3080440
• 负责人: DAVID N SHAPIRO
• 金额: $ 7.32万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080440
• 关键词: T cell receptor; antireceptor antibody; autoimmune disorder; autoradiography; calcium channel blockers; calcium metabolism; clone cells; cyclic AMP; cyclic GMP; guinea pigs; helper T lymphocyte; histocompatibility antigens; hybridomas; immunochemistry; immunoglobulin idiotypes; immunoregulation; insulin dependent diabetes mellitus; insulin receptor; interleukin 2; laboratory mouse; laboratory rabbit; laboratory rat; leukocyte activation /transformation; macrophage; monoclonal antibody; phosphorylation; protein kinase; radiotracer

The objectives of this project are to determine the structure and function of the T-cell immune recptor for insulin and to ascertain how its modulation affects antigen specific T-cell activation. These objectives will be accomplished by using established T-cell clones and newly generated T-cell hybridomas reactive with bovine insulin to prepare antibodies that specifically recognize the T-cell immune receptor. These anti-receptor reagents will then be used to invessigate and modulate the biochemical and physiological events which accompany antigen-specific activation of T cells and culminate in final T-cell effector function. The biochemical and functional characterization of the T-cell receptor and the intracellular events it modulates will be critical to our understanding of the factors controlling the antigen-specific response of this immunoregulatory cell. Aberrant regulation of the immune system has been implicated in the etiology of Type I diabetes mellitus and such therapeutic problems as insulin "allergy" and the development of insulin antibodies. Delineation of the mechanism and consequences of immune T-cell recognition of insulin will allow strategies for specific immunotherapy in diabetes.

这个项目的目标是确定结构和功能