RECEPTOR INDUCED ACTIVATION IN LYMPHOID CELLS

淋巴细胞中受体诱导的激活

• 批准号: 3466574
• 负责人: DAVID N SHAPIRO
• 金额: $ 3.73万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3466574
• 关键词: B lymphocyte; T cell receptor; T lymphocyte; antibiotics; antibody; autoradiography; biological polymorphism; calcium; chemical binding; cycloheximide; cyclosporines; cytokine receptors; gel electrophoresis; gene expression; genetic library; genetic manipulation; genetic transcription; helper T lymphocyte; hybridomas; immunoregulation; interferons; ionophores; isoquinolines; laboratory mouse; laboratory rabbit; leukocyte activation /transformation; lymphokines; macrophage; mitogens; molecular cloning; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; phorbols; phospholipase C; protein biosynthesis; protein kinase C; protein sequence; radiotracer; suppressor T lymphocyte; tissue /cell culture

The objectives of this research program are to study the sequence of cellular and molecular events initiated by antigen receptor perturbation and which culminate in T cell activation. These objectives will be accomplished by the techniques of differential nucleic acid hybridization in order to isolate genes that are transcriptionally active as a result of antigen receptor-induced activation. The development of cDNA probes from these genes will allow detailed analysis of T cell activation kinetics and stimulus-response coupling in this critical immunoregulatory cell as well as the identification of previously uncharacterized lymphokines. Aberrant and inappropriate T cell activation has a central role in the etiology of a variety of autoimmune, neoplastic, and hematologic disorders. Delineation of the mechanisms and consequences of immune T cell recognition of antigen will permit the development of novel strategies for specific immunotherapy based on a more complete understanding of the T cell activation sequence.

本研究计划的目标是研究 由抗原受体引发的细胞和分子事件 干扰并最终导致T细胞活化。 这些 目标将通过差异化技术来实现 核酸杂交，以分离 作为抗原受体诱导的结果， activation. 这些基因cDNA探针的开发 将允许详细分析T细胞活化动力学， 在这个关键的免疫调节细胞中的刺激-反应偶联 以及识别以前没有特征的 淋巴因子 异常和不适当的T细胞活化在以下方面具有核心作用： 各种自身免疫性、肿瘤性和 血液系统疾病 机制的界定和 免疫T细胞识别抗原的结果将允许 特异性免疫治疗新策略的开发 基于对T细胞活化的更全面理解， 顺序