REGULATION OF INTESTINAL ENZYME SYNTHESIS

肠道酶合成的调节

• 批准号: 3081332
• 负责人: RICARDO O CASTILLO
• 金额: $ 6.57万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3081332
• 关键词: O glycosidase; beta fructofuranosidase; enzyme induction /repression; enzyme substrate; growth /development; infant animal; laboratory rat; mature animal; membrane activity; nutrition related tag; organelles; parenteral feedings; protein biosynthesis; small intestines; tube feeding; weanling animal

The oligosaccharidases lactase and sucrase-dextrinase undergo dramatic alterations during development of the mammalian small intestine. In order to investigate the mechanisms relating to these dynamic events, the synthesis and membrane-associated assembly of these two enzymes will be studied in the maturing rat. Antibodies monospecific for lactase and sucrase-dextrinase will be used in combination with advanced techniques for intestinal perfusion and isolation of cellular organelles well established in the sponsor's laboratory to investigate molecular level events in the in vivo synthesis and assembly of these enzymes in the normally developing intestine. With these studies as a basis, regulation of the developmental alterations of lactase and sucrase-dextrinase by specific intraluminal carbohydrate substrates will be investigated at the molecular level using the above techniques in combination with a unique experimental model developed by the candidate. This model, in which nutrients adequate for normal growth are intravenously administered to infant animals prior to and during the weaning period, makes possible investigation of specific intraluminally administered nutrients without introduction of artifact due to malnutrition. These studies should generate precise information on regulation of synthesis and assembly of lactase and sucrase-dextrinase in the developing intestine and the mechanisms by which these enzymes are modulated by luminal nutrients during intestinal maturation.

寡糖酶、乳糖酶和蔗糖酶-糊精酶经过 哺乳动物小体发育过程中发生的巨大变化 肠。 为了研究相关机制 这些动态事件、合成和膜相关 这两种酶的组装将在成熟过程中进行研究 鼠。 乳糖酶和蔗糖酶-糊精酶单特异性抗体 将与先进技术结合使用 肠道灌注和细胞器分离良好 在申办者的实验室中建立以研究分子 这些物质的体内合成和组装过程中的水平事件 正常发育的肠道中的酶。 以这些研究为基础，发育调节 通过特定的方法改变乳糖酶和蔗糖酶-糊精酶 腔内碳水化合物底物将在 分子水平上使用上述技术结合 候选人开发的独特实验模型。 这 模型，其中足够正常生长的营养物质是 在之前和期间对幼年动物进行静脉注射 断奶期，使得可以对具体情况进行调查 管腔内给予营养物，无需引入 由于营养不良而造成的神器。 这些研究应该产生有关监管的准确信息 乳糖酶和蔗糖糊精酶的合成和组装 发育中的肠道以及这些酶的机制 在肠道成熟过程中受腔内营养物质的调节。