REGULATION OF INTESTINAL ENZYME SYNTHESIS

寡糖酶、乳糖酶和蔗糖-糊精酶 小型哺乳动物发育过程中的戏剧性变化 肠子。为了研究与之相关的机制 这些动态事件的合成与膜相关 这两种酶的组装将在成熟的 老鼠。乳糖酶和蔗糖酶-糊精酶单特异性抗体 将与高级技术相结合用于 肠道灌流和细胞器分离良好 在赞助商的实验室建立，以研究分子 它们在体内合成和组装中的水平事件 正常发育的肠道中的酶。 在这些研究的基础上，调节儿童的发展 乳糖酶和蔗糖酶-糊精酶的特异性变化 腔内碳水化合物底物将在 分子水平使用上述技术结合一个 候选人开发的独一无二的实验模型。这 模型，在该模型中，足够正常生长的养分 给幼年动物静脉注射之前和期间 断奶期，使具体的调查成为可能 铝内注射营养素而不引入 由于营养不良造成的艺术品。 这些研究应该产生关于监管的准确信息 乳糖酶和蔗糖酶-糊精酶的合成与组装 肠道发育及这些酶的作用机制 在肠道成熟过程中受到肠腔营养物质的调节。

The oligosaccharidases lactase and sucrase-dextrinase undergo dramatic alterations during development of the mammalian small intestine. In order to investigate the mechanisms relating to these dynamic events, the synthesis and membrane-associated assembly of these two enzymes will be studied in the maturing rat. Antibodies monospecific for lactase and sucrase-dextrinase will be used in combination with advanced techniques for intestinal perfusion and isolation of cellular organelles well established in the sponsor's laboratory to investigate molecular level events in the in vivo synthesis and assembly of these enzymes in the normally developing intestine. With these studies as a basis, regulation of the developmental alterations of lactase and sucrase-dextrinase by specific intraluminal carbohydrate substrates will be investigated at the molecular level using the above techniques in combination with a unique experimental model developed by the candidate. This model, in which nutrients adequate for normal growth are intravenously administered to infant animals prior to and during the weaning period, makes possible investigation of specific intraluminally administered nutrients without introduction of artifact due to malnutrition. These studies should generate precise information on regulation of synthesis and assembly of lactase and sucrase-dextrinase in the developing intestine and the mechanisms by which these enzymes are modulated by luminal nutrients during intestinal maturation.