REGULATION OF INTESTINAL ENZYME SYNTHESIS

寡糖酶乳糖酶和蔗糖酶-糊精酶经历 在哺乳动物的小细胞发育过程中， 肠子 为了研究与 这些动态事件，合成和膜相关 这两种酶的组装将在成熟过程中进行研究。 大鼠 乳糖酶和蔗糖酶-糊精酶单特异性抗体 将与先进技术结合使用， 肠灌流和细胞器分离良好 在申办方实验室建立，以研究分子 在体内合成和组装这些水平的事件 正常发育的肠道中的酶。 以这些研究为基础， 乳糖酶和蔗糖酶-糊精酶的改变 管腔内碳水化合物底物将在 分子水平上使用上述技术结合 候选人开发的独特实验模型。 这 模型，其中正常生长所需的营养物质是 在给药前和给药期间， 断奶期，使可能的调查，具体 管腔内给予的营养物， 是营养不良造成的 这些研究应提供关于以下方面的精确信息： 乳糖酶和蔗糖酶-糊精酶的合成与组装 以及这些酶 在肠成熟过程中受到肠腔营养素的调节。

The oligosaccharidases lactase and sucrase-dextrinase undergo dramatic alterations during development of the mammalian small intestine. In order to investigate the mechanisms relating to these dynamic events, the synthesis and membrane-associated assembly of these two enzymes will be studied in the maturing rat. Antibodies monospecific for lactase and sucrase-dextrinase will be used in combination with advanced techniques for intestinal perfusion and isolation of cellular organelles well established in the sponsor's laboratory to investigate molecular level events in the in vivo synthesis and assembly of these enzymes in the normally developing intestine. With these studies as a basis, regulation of the developmental alterations of lactase and sucrase-dextrinase by specific intraluminal carbohydrate substrates will be investigated at the molecular level using the above techniques in combination with a unique experimental model developed by the candidate. This model, in which nutrients adequate for normal growth are intravenously administered to infant animals prior to and during the weaning period, makes possible investigation of specific intraluminally administered nutrients without introduction of artifact due to malnutrition. These studies should generate precise information on regulation of synthesis and assembly of lactase and sucrase-dextrinase in the developing intestine and the mechanisms by which these enzymes are modulated by luminal nutrients during intestinal maturation.