REGULATION OF INTESTINAL ENZYME SYNTHESIS

肠道酶合成的调节

• 批准号: 3081333
• 负责人: RICARDO O CASTILLO
• 金额: $ 6.59万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3081333
• 关键词: O glycosidase; beta fructofuranosidase; enzyme induction /repression; enzyme substrate; growth /development; infant animal; laboratory rat; mature animal; membrane activity; nutrition related tag; organelles; parenteral feedings; protein biosynthesis; small intestines; tube feeding; weanling animal

The oligosaccharidases lactase and sucrase-dextrinase undergo dramatic alterations during development of the mammalian small intestine. In order to investigate the mechanisms relating to these dynamic events, the synthesis and membrane-associated assembly of these two enzymes will be studied in the maturing rat. Antibodies monospecific for lactase and sucrase-dextrinase will be used in combination with advanced techniques for intestinal perfusion and isolation of cellular organelles well established in the sponsor's laboratory to investigate molecular level events in the in vivo synthesis and assembly of these enzymes in the normally developing intestine. With these studies as a basis, regulation of the developmental alterations of lactase and sucrase-dextrinase by specific intraluminal carbohydrate substrates will be investigated at the molecular level using the above techniques in combination with a unique experimental model developed by the candidate. This model, in which nutrients adequate for normal growth are intravenously administered to infant animals prior to and during the weaning period, makes possible investigation of specific intraluminally administered nutrients without introduction of artifact due to malnutrition. These studies should generate precise information on regulation of synthesis and assembly of lactase and sucrase-dextrinase in the developing intestine and the mechanisms by which these enzymes are modulated by luminal nutrients during intestinal maturation.

低聚糖酶乳糖酶和蔗糖糊化酶进行

项目成果

Mechanism of maturational decline of rat intestinal lactase-phlorizin hydrolase.

大鼠肠道乳糖酶-根皮苷水解酶成熟衰退的机制。

• DOI: 10.1042/bj2820107
• 发表时间: 1992
• 期刊: The Biochemical journal
• 作者: Tsuboi,KK;Kwong,LK;Sunshine,P;Castillo,RO
• 通讯作者: Castillo,RO

Altered maturation of small intestinal function in the absence of intraluminal nutrients: rapid normalization with refeeding.

在缺乏腔内营养物的情况下，小肠功能的成熟发生改变：通过重新喂养快速正常化。

• DOI: 10.1093/ajcn/53.2.558
• 发表时间: 1991
• 期刊: The American journal of clinical nutrition
• 作者: Castillo,RO;Feng,JJ;Stevenson,DK;Kwong,LK
• 通讯作者: Kwong,LK

Synthesis and accumulation of protein and carbohydrases along the rat villus column.

蛋白质和糖酶沿着大鼠绒毛柱的合成和积累。

• DOI: 10.1097/00005176-199110000-00001
• 发表时间: 1991
• 期刊: Journal of pediatric gastroenterology and nutrition
• 影响因子: 2.9
• 作者: Castillo,RO;Kwong,LK;Tsuboi,KK
• 通讯作者: Tsuboi,KK

Alterations in postnatal intestinal function during chronic hypoxemia.

慢性低氧血症期间产后肠道功能的改变。

• DOI: 10.1203/00006450-199203000-00008
• 发表时间: 1992
• 期刊: Pediatric research
• 影响因子: 3.6
• 作者: Bernstein,D;Bell,JG;Kwong,L;Castillo,RO
• 通讯作者: Castillo,RO

Maturation of jejunoileal gradients in rat intestine: the role of intraluminal nutrients.

大鼠肠道空肠油梯度的成熟：腔内营养物的作用。

• DOI: 10.1159/000243891
• 发表时间: 1992
• 期刊: Biology of the neonate
• 作者: Castillo,RO;Feng,JJ;Stevenson,DK;Kwong,LK
• 通讯作者: Kwong,LK