NOVEL EXTRACELLULAR MATRIX PROTEIN IN FETAL HEART

胎儿心脏中的新型细胞外基质蛋白

• 批准号: 3083106
• 负责人: James D BRISTOW
• 金额: $ 7.29万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3083106
• 关键词: Escherichia coli; antiserum; biological models; cell adhesion; developmental genetics; early embryonic stage; embryo /fetus; extracellular matrix proteins; fibronectins; gene expression; heart; immunocytochemistry; in situ hybridization; laboratory rat; molecular cloning; muscle cells; newborn animals; nucleic acid sequence; polymerase chain reaction; tissue /cell culture

The research plan proposes the cloning, characterization of expression and initial functional analysis of a novel extracellular matrix protein in the developing rat heart. The gene encoding this protein (gene X) initially was identified in the human fetal adrenal by the candidate while working in the sponsors laboratory. Happily, recent observations have shown that there is significant gene X expression in human fetal heart. Protein homology analysis has demonstrated significant homology between the gene X protein and well-characterized extracellular matrix proteins, including fibronectin and tenascin, both of which appear to be important in cardiac morphogenesis. Progress on the human gene product has been slowed by the lack of availability of high quality human tissues for analysis. There is strong presumptive evidence that gene X exists and is expressed in fetal rat heart. A more complete understanding of the function of the gene X protein mandates development of a model animal system, a goal which this proposal will accomplish.

该研究计划提出了表达的克隆、表征和 一种新型细胞外基质蛋白的初步功能分析 发育中的大鼠心脏。 最初编码该蛋白质的基因（基因 X） 候选人在工作时在人类胎儿肾上腺中发现了 赞助者实验室。 令人高兴的是，最​​近的观察表明 人类胎儿心脏中存在显着的X基因表达。 蛋白质 同源性分析表明基因 X 之间具有显着的同源性 蛋白质和充分表征的细胞外基质蛋白，包括 纤连蛋白和腱蛋白，两者似乎对心脏都很重要 形态发生。 人类基因产品的进展已经放缓 缺乏可供分析的高质量人体组织。 有 强有力的推定证据表明基因 X 存在并在胎儿中表达 老鼠的心。 更全面地了解 X 基因的功能 蛋白质要求开发模型动物系统，这一目标 提案将会完成。