PATHOGENIC ROLES OF THE ALVEOLAR MACROPHAGE IN ASTHMA

哮喘是一种具有重要临床和经济意义的疾病， 折磨着大约900万年轻的、原本健康的人。 炎症是哮喘的一个重要方面，但许多 炎症的潜在决定因素还没有研究过。 肺泡巨噬细胞(AM)的作用将由 这些建议的研究。这些细胞很可能对 气道炎症和高反应性的产生 能量氧物种(HEOS)，释放趋化因子 炎性细胞，如嗜酸性粒细胞和中性粒细胞，以及 阐述促进组胺释放的因素。过敏症 哮喘受试者将在治疗前后进行研究 用对它们敏感的抗原进行支气管炎(BP)， AM经支气管肺泡灌洗恢复。HEOS代谢 将通过超氧化物的生化测定来确定 和过氧化氢以及鲁米诺增强剂 化学发光反应。趋化活性物质的释放 粒细胞将通过标准的微趋化性来确定。 测定，以及AM衍生的组胺释放活性 组胺的荧光分析。过敏性哮喘的结果 BP后的患者将与正常患者进行比较 对照和过敏性鼻炎患者，以及自己的价值观 在BP之前。

Asthma is a disease of great clinical and economic importance, afflicting some nine million young, otherwise healthy individuals. Inflammation is an important aspect of asthma, but many potential determinants of inflammation have not been studied. Roles of the alveolar macrophage (AM) will be determined by these proposed studies. It is likely that these cells contribute to airway inflammation and hyperreactivity by generation of high energy oxygen species (HEOS), release of chemotactins for inflammatory cells such as eosinophils and neutrophils, and elaboration of factors promoting histamine release. Allergic asthmatic subjects will be studied before and after bronchoprovaction (BP) with antigen to which they are sensitive, and AM recovered by bronchoalveolar lavage. HEOS metabolism will be established by biochemical determinations of superoxide and hydrogen peroxide in addition to luminol-enhanced chemiluminescence response. Release of chemotactins for granulocytes will be determined by standard microchemotaxis assays, and AM-derived histamine releasing activity by fluorimetric analysis of histamine. Results from allergic asthma patients after BP will be compared to those obtained from normal controls and allergic rhinitis patients, and to their own values prior to BP.