CREATIVE KINASE FLUX IN VIVO
CREATIVE KINASE FLUX IN VIVO
批准号:
3082006
负责人:
JOHN A BITTL
金额:
$6.51万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-04-05 至 1990-04-04
关键词:
adenosine triphosphate bioenergetics cellular respiration creatine kinase electrodes electrophoresis enzyme mechanism heart heart contraction heart function laboratory rat mitochondria myocardial ischemia /hypoxia myocardium norepinephrine nuclear magnetic resonance spectroscopy oxidative phosphorylation oxygen consumption striated muscles
中文摘要
磁化转移的~(31)P-NMR技术可用于研究
肌酸激酶反应的细胞内动力学明确在
离体大鼠心脏和活体大鼠后肢骨骼肌
大鼠 本研究项目的目的是表征肌酸
体内激酶反应。 验证研究将侧重于法规
肌酸激酶活性随pH值、温度和磷酸肌酸
内容被改变了。 前两个实验将在
用不同pH值或不同pH值的培养基灌注离体大鼠心脏,
温度 后一项实验将在离体心脏中进行
在老鼠的骨骼肌中,
1% β-胍基丙酸6周。
我们实验室的初步研究表明,肌酸激酶流量
在离体的、氧合良好的大鼠心脏中,
性能、耗氧量和线粒体呼吸,因此
为磷酸肌酸穿梭提供了体内证据。 进一步
研究将评估正性肌力药的作用
去甲肾上腺素对肌酸激酶流量、心脏性能和
线粒体呼吸在离体大鼠心脏。 此外,关系
肌肉收缩和肌酸激酶流量之间的关系将在
肌酸激酶相关性研究
通量,用31 P-NMR表面线圈测量,对肌肉的速率
刺激股神经引起的收缩。
采用~(31)P-NMR测定缺氧大鼠肌酸激酶的流量
心 流量与心脏功能和氧耗的相关性
将决定肌酸激酶流量是否与
高能量磷酸盐利用率,如心脏中所反映的,
性能,或线粒体ATP生产率,估计
通过氧气消耗。 其他研究将确定
肌酸激酶流量、心脏性能和心脏耗氧量
缺血后再灌注,以确定是否
缺血后大鼠心脏的心脏功能受到抑制,
线粒体呼吸和肌酸之间的中断或解偶联
激酶通量
除了提供关于体内酶活性的关键信息外,
和肌肉生物能量学,这里提出的实验将有助于
了解高能量磷酸盐代谢所需的
31 P-NMR的临床应用。
英文摘要
The 31P-NMR technique of magnetization transfer can be used to investigate
the intracellular kinetics of the creatine kinase reaction explicitly in
isolated rat heart and in skeletal muscle of the hindlimb of the living
rat. The purpose of this research project is to characterize the creatine
kinase reaction in vivo. Validation studies will focus on the regulation
of creatine kinase activity as pH, temperature and creatine phosphate
content are altered. The former two experiments will be performed in
isolated rat hearts perfused with media at various pH values or at various
temperatures. The latter experiment will be carried out in isolated hearts
and in the skeletal muscle of rats that have been fed a diet that contains
1% Beta-guanidinoproprionic acid for 6 weeks.
Preliminary studies from our laboratory suggest that creatine kinase flux
in the isolated, well-oxygenated rat hearts increases with cardiac
performance, oxygen consumption and mitochondrial respiration, thus
providing in vivo evidence for the creatine phosphate shuttle. Further
investigation will evaluate the effect of the inotropic agent
norepinephrine on creatine kinase flux, cardiac performance and
mitochondrial respiration in the isolated rat heart. Also, the relation
between muscle contraction and creatine kinase flux will be investigated in
the quadriceps muscle of the living rat by correlating creatine kinase
flux, measured with a 31P-NMR surface coil, to the rate of muscle
contraction from stimulation of the femoral nerve.
31P-NMR will be used to measure creatine kinase flux in the hypoxic rat
heart. Correlating flux to cardiac performance and to oxygen consumption
in hypoxic hearts will determine whether creatine kinase flux is coupled to
the rate of high-energy phosphate utilization, as reflected in cardiac
performance, or to the rate of mitochondrial ATP production, as estimated
by oxygen consumption. Other studies will identify the relationship among
creatine kinase flux, cardiac performance and oxygen consumption in hearts
that have been reperfused after ischemia in order to determine whether
cardiac performance in post-ischemic rat hearts is depressed because of a
disruption or uncoupling between mitochondrial respiration and creatine
kinase flux.
In addition to providing critical information about enzyme activity in vivo
and muscle bioenergetics, the experiments proposed here will contribute to
the understanding of high-energy phosphate metabolism needed for the
clinical application of 31P-NMR.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
Peak left ventricular pressure during percutaneous aortic balloon valvuloplasty: clinical and echocardiographic correlations.
经皮主动脉球囊瓣膜成形术期间左心室峰值压力:临床和超声心动图相关性。
DOI:
10.1016/0735-1097(89)90063-6
发表时间:
1989
期刊:
Journal of the American College of Cardiology
影响因子:
24
作者:
[Bittl,JA, Bhatia,SJ, Plappert,T, Ganz,P, StJohnSutton,MG, Selwyn,AP]
通讯作者:
Selwyn,AP
CORONARY LASER ANGIOPLASTY
-
批准号:6252462
-
项目类别:
-
资助金额:$4.57万
-
财政年份:1997
-
负责人:JOHN A BITTL
-
依托单位:
GR II INTRACORONARY STENT TRIAL
-
批准号:6281899
-
项目类别:
-
资助金额:$4.73万
-
财政年份:1997
-
负责人:JOHN A BITTL
-
依托单位:
ACS MULTILINK CORONARY STENT TRIAL
-
批准号:6281898
-
项目类别:
-
资助金额:$4.73万
-
财政年份:1997
-
负责人:JOHN A BITTL
-
依托单位:
CREATIVE KINASE FLUX IN VIVO
-
批准号:3082004
-
项目类别:
-
资助金额:$6.45万
-
财政年份:1985
-
负责人:JOHN A BITTL
-
依托单位:
CREATIVE KINASE FLUX IN VIVO
-
批准号:3082005
-
项目类别:
-
资助金额:$6.48万
-
财政年份:1985
-
负责人:JOHN A BITTL
-
依托单位:
CREATIVE KINASE FLUX IN VIVO
-
批准号:3082002
-
项目类别:
-
资助金额:$6.56万
-
财政年份:1985
-
负责人:JOHN A BITTL
-
依托单位:
CREATIVE KINASE FLUX IN VIVO
-
批准号:3082003
-
项目类别:
-
资助金额:$6.56万
-
财政年份:1985
-
负责人:JOHN A BITTL
-
依托单位:
ACS MULTILINK CORONARY STENT TRIAL
-
批准号:6121358
-
项目类别:
-
资助金额:$5.27万
-
财政年份:--
-
负责人:JOHN A BITTL
-
依托单位:
GR II INTRACORONARY STENT TRIAL
-
批准号:6121359
-
项目类别:
-
资助金额:$5.27万
-
财政年份:--
-
负责人:JOHN A BITTL
-
依托单位:
海外基金