Elucidating the post-transcriptional regulation of mast cell proteases

阐明肥大细胞蛋白酶的转录后调控

• 批准号: DP150102153
• 负责人: Prof Phil Hansbro
• 金额: $ 30.62万
• 依托单位: The University of Newcastle
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-06-30 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150102153
• 关键词: Elucidating; post; transcriptional; regulation; mast

Mast cells (MCs) are immune cells that protect against pathogens but may induce deleterious inflammation. MC function is mediated by specific proteases that are pre-formed and stored in granules. These proteases have unique yet poorly understood mechanisms of regulation. The aim of the project is to use a novel suite of molecular tools and genetically modified mice to identify the critical regions of transcripts that post-transcriptionally regulate the production and storage of these proteins. The project aims to identify the RNA binding proteins, microRNAs and other novel factors that also regulate them. This is expected to elucidate the post-transcriptional mechanisms of regulation of MC proteases.

肥大细胞（MC）是免疫细胞，可抵御病原体，但可能会诱导有害的炎症。MC功能由预先形成并储存在颗粒中的特定蛋白酶介导。这些蛋白酶具有独特但知之甚少的调节机制。该项目的目的是使用一套新型分子工具和转基因小鼠来识别转录后调节这些蛋白质产生和储存的转录物的关键区域。该项目旨在识别RNA结合蛋白，microRNA和其他调节它们的新因子。这有望阐明MC蛋白酶调控的转录后机制。