BIOENERGETIC MECHANISMS

生物能量机制

• 批准号: 3087472
• 负责人: STEVAN ZIMMER
• 金额: $ 7.49万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087472
• 关键词: acidity /alkalinity; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; bioenergetics; cardiac output; creatine; creatine kinase; dobutamine; enzyme substrate; glucose; heart contraction; heart disorder diagnosis; heart failure; heart function; heart metabolism; hemodynamics; high energy compound; hydrogen; infarct; insulin; ischemia; lactates; mitochondria; model design /development; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; oxidative phosphorylation; oxygen consumption; perfusion; phosphates; pyruvates; stable isotope; taurine; tissue /organ preservation

Changes in high energy phosphate (HEP) metabolism has been implicated as a causative factor in the pathophysiologic conditions of post-ischemic mechanical dysfunction ("stunned myocardium") and post-infarction heart failure. To examine this possible relationship, we are applying the emerging methodology of NMR spectroscopy in an isolated heart model to the study of myocardial bioenergetics in these conditions. 31p and 1H NMR spectroscopy offers the unique advantage of noninasive, real-time assessment of dynamic changes in intracellular metabolites and HEP fluxes in intact organ systems. While the understanding of cardiac energy metabolism during normal and pathologic conditions has been greatly advanced using traditional techniques, NMR spectroscopy offers the potential for more detailed understanding of the dynamics of cellular bioenergetics in these pathophysiologic conditions.

高能磷酸盐(HEP)代谢的变化 与病理生理学中的致病因素有关 缺血后机械功能障碍的情况(“晕厥” 心肌“)和心肌梗塞后的心力衰竭。 可能的关系，我们正在应用新兴的方法论 核磁共振波谱在离体心模型中的应用 在这些条件下的心肌生物能量学。31P和1H核磁共振 光谱学提供了非侵入性、实时 评估细胞内代谢物的动态变化和 HEP在完整的器官系统中的通量。在理解的同时 正常和病理状态下的心脏能量代谢 使用传统技术，条件得到了极大的改善， 核磁共振光谱学提供了更详细的 对这些细胞生物能量学动力学的理解 病理生理条件。