BIOENERGETIC MECHANISMS

生物能量机制

• 批准号: 3087474
• 负责人: STEVAN ZIMMER
• 金额: $ 6.99万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087474
• 关键词: acidity /alkalinity; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; bioenergetics; cardiac output; creatine; creatine kinase; dobutamine; enzyme inhibitors; enzyme substrate; glucose; heart contraction; heart disorder diagnosis; heart failure; heart function; heart metabolism; hemodynamics; high energy compound; hydrogen; infarct; insulin; ischemia; laboratory rat; lactates; mitochondria; model design /development; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; oxidative phosphorylation; oxygen consumption; perfusion; phosphates; pyruvates; stable isotope; taurine; tissue /organ preservation

Changes in high energy phosphate (HEP) metabolism has been implicated as a causative factor in the pathophysiologic conditions of post-ischemic mechanical dysfunction ("stunned myocardium") and post-infarction heart failure. To examine this possible relationship, we are applying the emerging methodology of NMR spectroscopy in an isolated heart model to the study of myocardial bioenergetics in these conditions. 31p and 1H NMR spectroscopy offers the unique advantage of noninasive, real-time assessment of dynamic changes in intracellular metabolites and HEP fluxes in intact organ systems. While the understanding of cardiac energy metabolism during normal and pathologic conditions has been greatly advanced using traditional techniques, NMR spectroscopy offers the potential for more detailed understanding of the dynamics of cellular bioenergetics in these pathophysiologic conditions.

高能磷酸盐（HEP）代谢的变化已被证实。 作为病理生理学的致病因素， 缺血后机械功能障碍的状况（“休克 心肌”）和梗死后心力衰竭。 审查这个 可能的关系，我们正在应用新兴的方法， 在离体心脏模型中的核磁共振波谱研究 在这些条件下的心肌生物能量学。 31p和1H NMR 光谱学提供了独特的优势， 评估细胞内代谢物的动态变化， 完整器官系统中的HEP通量。 虽然理解 正常和病理状态下心脏能量代谢 使用传统技术的条件已经大大改善， NMR光谱提供了更详细的 了解这些细胞生物能量学的动态， 病理生理条件。