The core inflammasome as a model for caspase activation

作为半胱天冬酶激活模型的核心炎症体

• 批准号: DP180103983
• 负责人: A/Prof Katryn Stacey
• 金额: $ 38.2万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP180103983
• 关键词: core; inflammasome; model; caspase; activation

This project aims to change the paradigm for the structure of the active inflammasome. Inflammasomes activate caspases, enzymes central to cell death and inflammatory processes. The current concept of inflammasomes is that caspases are recruited into a single massive protein complex seen as a “speck” in the cell. This project proposes the speck is a terminal stage, after the major enzymatic activity is over. This project aims to purify smaller early stage inflammasome complexes, for structural analysis. The outcome will be a clearer understanding of processes of caspase activation and inflammasome formation. This will provide significant benefits, such as improve our understanding of processes of cell death and innate immunity, and train students.

该项目旨在改变活性炎症体结构的范式。炎症小体激活半胱天冬酶，这是细胞死亡和炎症过程的核心酶。目前炎症小体的概念是，半胱天冬酶被募集到单个巨大的蛋白质复合物中，被视为细胞中的“斑点”。该项目提出，斑点是主要酶活性结束后的最后阶段。该项目旨在纯化较小的早期炎症体复合物，用于结构分析。结果将是对半胱天冬酶激活和炎症小体形成过程的更清晰的了解。这将带来显着的好处，例如提高我们对细胞死亡和先天免疫过程的理解，并培训学生。