STUDIES IN AUTOLOGOUS BONE MARROW TRANSPLANTATION

这项资助解决了自体骨髓的作用。 急性白血病、骨髓瘤和弥漫性大细胞的移植 淋巴瘤联合大剂量化疗和全量化疗 身体照射。由于这些是以骨髓为基础的疾病， 先洗后除恶性细胞的问题 移植是显而易见的。两个新的分离程序 已被开发，体外免疫胶体分离和 药理上的肿瘤细胞减少。我们建议研究 分离技术对造血功能的影响 每种分离的重建能力和效果 以肿瘤复发的时间来衡量。检测系统，用于 白血病细胞已经用分子探针和 将用于监测骨髓中的白血病细胞 污染按清洗方法进行。定义明确 肿瘤标记物将用于骨髓瘤的移植。

This grant addresses the role of autologous bone marrow transplantation in acute leukemia, myeloma and diffuse large cell lymphoma when combined with high-dose chemotherapy and total body irradiation. As these are bone marrow based diseases, the question of purging to remove malignant cells before transplantation is evident. Two new separation procedures have been developed, in vitro immunocolloid separation and pharmacological tumor cell reduction. We propose to study the effect of the separation technique on hematopoietic reconstruction capacity and the efficacy of each separation as measured by time to tumor recurrence. Detection systems for leukemic cells have been developed using molecular probes and will be applied for monitoring bone marrow for leukemic cell contamination according to method of purging. Well defined tumor markers will be employed in transplants in myeloma.