STUDIES IN AUTOLOGOUS BONE MARROW TRANSPLANTATION

这项拨款解决了自体骨髓的作用 急性白血病、骨髓瘤和弥漫性大细胞白血病移植 淋巴瘤时，结合高剂量化疗和总 身体辐射 由于这些都是基于骨髓的疾病， 清除恶性细胞的问题， 移植是显而易见的。 两个新的分离程序 体外免疫胶体分离和 药理学肿瘤细胞减少。 我们建议研究 分离技术对造血功能的影响 重建能力和每次分离的功效， 通过肿瘤复发的时间来测量。 探测系统 已经使用分子探针开发了白血病细胞， 将用于监测骨髓白血病细胞 根据清洗方法的污染。 明确定义 肿瘤标记物将用于骨髓瘤的移植。

This grant addresses the role of autologous bone marrow transplantation in acute leukemia, myeloma and diffuse large cell lymphoma when combined with high-dose chemotherapy and total body irradiation. As these are bone marrow based diseases, the question of purging to remove malignant cells before transplantation is evident. Two new separation procedures have been developed, in vitro immunocolloid separation and pharmacological tumor cell reduction. We propose to study the effect of the separation technique on hematopoietic reconstruction capacity and the efficacy of each separation as measured by time to tumor recurrence. Detection systems for leukemic cells have been developed using molecular probes and will be applied for monitoring bone marrow for leukemic cell contamination according to method of purging. Well defined tumor markers will be employed in transplants in myeloma.