PROPOSED NATIONAL VIBRATING PROBE FACILITY

我们建议在海洋生物中心建立一个国家设施 实验室(MBL)，包含基于 超灵敏的振动探头。这将使 生物医学社区高效地绘制和测量电场 围绕着不同的生命系统；并因此决定了模式和(部分) 穿过这些系统的离子流的性质。第一 安装将包含两个螺杆探头系统以及 关联的视频和计算机系统，以高效地减少、显示和 记录测量的字段。变位探头可同时提供 在任意点测量场的两个分量，并应允许 恒定流过河床的有效探索 微小细胞突起周围的含水介质，如突起以及 较大的细胞和组织，如卵子和肌肉。在适当的时候，我们 建议开发更多版本的振动探头，包括 空中一号，测量空中系统(如某些昆虫)附近的区域 鸡蛋、真菌、哺乳动物皮肤等)和三维模型。我们计划 开展各种合作研究项目，特别是关于 内生流在某些海洋中建立模式中的作用 卵子和哺乳动物卵裂球的极化，以及内源性的作用 末端电流在控制神经生长中的作用。我们希望满足以下需求 非常广泛的生物医学用户，包括对 通过肌肉、神经和血细胞的内源性电流，通过各种 卵和胚胎，通过生长植物细胞，发挥各种功能 上皮细胞、再生肢体和神经残端等。我们也一样 计划提供有关使用振动探头的适当培训。

We propose to establish a national facility at the Marine Biological Laboratory (MBL) containing advanced instrumentation based upon the ultrasensitive vibrating probe. This will enable various members of the biomedical community to efficiently map and measure the electrical fields around various living systems; and thus determine the pattern and (in part) the nature of the ionic currents which traverse these systems. The first installation will contain two nutating probe systems together with associated video and computer systems to efficiently reduce, display, and record the fields which are measured. Nutating probes provide simultaneous measurements of two components of the field at any point and should allow the effective exploration of the steady currents which traverse the acqueous media around minute cellular processes such as neurites as well as larger cells and tissues such as eggs and muscles. In due course we propose to develop further versions of the vibrating probe including an aerial one to measure fields near aerial systems (such as certain insect eggs, fungi, mammalian skin, etc.), and a three dimensional one. We plan to carry out various collaborative research projects, particularly ones on the role of endogenous currents in establishing pattern in certain marine eggs and in polarizing mammalian blastomeres, and the role of endogenous tip currents in controlling nerve growth. We expect to serve the needs of a very wide variety of biomedical users including persons interested in endogenous currents through muscle, nerve and blood cells, through various eggs and embryos, through growing plant cells, various functioning epithelia, regenerating limbs and nerve stumps and so forth. We likewise plan to provide appropriate training in the use of the vibrating probe.