FREE CYTOSOLIC CALCIUM GRADIENTS IN EARLY DEVELOPMENT

早期发育中的游离细胞溶钙梯度

• 批准号: 3315969
• 负责人: LIONEL F JAFFE
• 金额: $ 10.31万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3315969
• 关键词: Dictyostelium; algae; cell differentiation; cytoplasm; early embryonic stage; egg /ovum; fluorescent dye /probe; image processing; mesoderm; plant growth /development; radiation detector; saltwater environment; visible light

The P.I. proposes to explore the role of steady free cytosolic calcium ion gradients in establishing pattern during early development. Such gradients have never been directly demonstrated. Nevertheless, a slowly growing but substantial body of indirect evidence indicates both their existence, their direction and their significance; while recent technical advances should now make it possible to directly detect and measure such gradients. The methods which are proposed to accomplish this involve the use of chemically modified aequorins and a so-called imaging photon detector as well as a conventional photon counting system. The living systems proposed for their exploration include the fucoid (brown algal) egg, the medaka fish egg, the sea urchin egg, the ascidian egg and the cellular slime mold, Dictyostelium. Where gradients can be measured, their developmental role will then be explored. This will be done by means intended to modify (or even suppress) calcium gradients and/or developmental pattern. These means will include the use of calcium buffer injections and imposed calcium ionophore gradients as well as lithium ions and other pattern-modifying agents as well as pattern mutants. The fundamental insights that may be gained in these studies should ultimately relate to those aspects of health which depend upon growth and development.

私家侦探建议探讨稳定的游离胞浆钙离子的作用， 在早期发育过程中建立模式的梯度。 这样的梯度 从未被直接证明过。 然而，一个缓慢增长的， 大量的间接证据表明它们的存在， 发展方向及其重要性;虽然最近的技术进步应 现在可以直接检测和测量这种梯度。 提出的实现这一点的方法涉及使用 化学修饰的水母发光蛋白和所谓的成像光子检测器， 以及常规光子计数系统。 提出的生命系统 他们的探索包括岩藻（褐藻）蛋，青鳉鱼， 卵、海胆卵、海鞘卵和细胞黏菌， 网骨藻 在梯度可以测量的地方，它们的发展作用将是 探讨了 这将通过旨在修改（甚至抑制） 钙梯度和/或发育模式。 这些手段将包括 使用钙缓冲液注射和施加的钙离子载体 梯度以及锂离子和其他图案改性剂， 以及模式突变体 从这些研究中可能获得的基本见解应该 最终与健康的那些方面有关，这些方面取决于生长， 发展