TISSUE LABORATORY CORE

这笔SCOR赠款的大多数项目都将不同程度地依赖于 关于培养的和分离的滑膜的可用性 微环境组件以及 新鲜和培养的滑膜T细胞制剂。此外，每个 人体组织、滑液或外周血液样本 需要登录、处理，如果是组织，则部分 为免疫组织学冷冻，为光照准备的部分 显微镜，以及为电子显微镜准备的部分。 单抗的制备和使用也需要 该中心的许多调查人员。此外，Project 5将使用 MRL小鼠关节炎模型并将需要组织切片 用于组织病理学和原位杂交分析的单抗 抗体技术，并最终培育出 小鼠滑膜细胞。在开发此应用程序时， 决定最经济有效的方法是 满足整个计划的这些需求就是将这些 活动。这一核心设施的主要理由 来自于高度劳动密集型的增长和 表征滑膜微环境成分，还 源于辛格博士在执行这些任务方面的特殊技能。 这个核心单元I的具体目标是培养滑膜细胞 并提供新鲜的巨噬细胞和T细胞样本 研究人员进行了功能研究，以建立滑膜组织 银行，以便提供新鲜或冷冻的滑膜组织样本 用于研究，并向研究人员提供组织切片 进行原位杂交研究，免疫荧光 化验，或光学显微镜分析。该核心设施是 被设计成组织培养细胞的重要来源 将向所有成员提供的组织切片 中锋。适当地使用这一设施将防止不必要的 重复的工作和费用，并将确保统一 所研究的细胞群体和组织。

Most projects of this SCOR grant will depend to varying degrees upon the availability of cultured and isolated synovial microenvironment components as well as on the availability of fresh and cultured synovial T cell preparations. Moreover, each human tissue, synovial fluid, or peripheral blood specimen will need to be logged in, processed, and in the case of tissue, portions frozen for immunohistology, portions prepared for light microscopy, and portions prepared for electron microscopy. Preparation and use of monoclonal antibodies are also required by many of the Center's investigators. In addition, Project 5 will use the MRL mouse model of arthritis and will require tissue sections for histopathology and in situ hybridization assays, monoclonal antibody technology, and eventually the techniques for growing murine synovial cells. In developing this application, it was decided that the most economical and efficacious approach to meet these needs for the entire program was to centralize these activities. The principal justification for this Core facility derives from the highly labor intensive nature of growing and characterizing synovial microenvironment components and also derives from Dr. Singer's special skills in performing these tasks. The Specific Aims of this Core Unit I are to culture synovial cells and to provide fresh samples of macrophages and T cells to investigators for functional studies, to establish a synovial tissue bank in order to provide fresh or frozen synovial tissue samples for studies, and to provide tissue sections to investigators performing in situ hybridization studies, immunofluorescent assays, or light microscopic analysis. This Core facility is designed to be an important source of tissue culture cells and tissue sections that will be available to all members of the Centers. Use of this facility appropriately will prevent needless duplication of effort and expense and will ensure uniformity of cell populations and tissues studied.