CORE--TISSUE CULTURE, MONOCLONAL ANTIBODY, HISTOPATHOLOGY, AND FLOW CYTOMETRY

该中心赠款的大多数项目都依赖并继续依赖于 根据培养的和分离的滑膜的可用性而变化 微环境组件以及新鲜和 培养的滑膜T细胞制剂。 核心基金提供一个 所有这些组织的中心来源，并已应用了特殊的 分离和生长滑膜衬里细胞所需的专业知识。 每个人体组织或外周血样本都要记录、处理， 并且，在组织的情况下，冷冻部分用于以后的培养， 免疫组织学、制备用于光学显微镜检查的部分和部分 用于电子显微镜检查。 单克隆抗体的制备和应用 该中心的许多研究人员和核心小组都需要抗体。 设施作为许多单克隆抗体的中心来源。 组织切片用于免疫荧光染色和原位 杂交分析对于许多研究是必要的， 对滑膜炎症的持续研究至关重要。 一个新 除核心设施外，还提供流式细胞仪设施 以及用于表型分析和淋巴细胞分离的专业知识。 细胞和培养的滑膜衬里细胞或唾液上皮细胞。 的 这一核心设施的主要理由来自高度 劳动密集型性质的增长和表征滑膜 微观环境组成部分和消除重复劳动， 个体研究者获得必要的细胞群， 组织研究。 第一核心单位的具体目标是： 滑膜细胞，并提供巨噬细胞和T细胞的新鲜样品， 功能研究的研究者，以维持滑膜组织库， 提供新鲜或冷冻滑膜组织样本用于研究， 组织切片给进行原位杂交研究的研究者， 免疫荧光测定或光显微镜分析，以提供 单克隆抗体的研究，并提供流式细胞仪设施 和专业知识。 该设施旨在成为一个 组织培养细胞和组织切片的重要来源， 向中心所有成员开放。

Most projects of this center grant have depended and continue to depend to varying on the availability of cultured and isolated synovial microenvironment components as well as the availability of fresh and cultured synovial T cell preparations. The Core Facility provides a central source for all of these tissues and has applied the special expertise necessary to isolation and growth of the synovial lining cells. Each human tissue or peripheral blood specimen is logged in, processed, and, in the case of tissue, portions frozen for later culture and for immunohistology, portions prepared for light microscopy, and portions prepared for electron microscopy. The preparation and use of monoclonal antibodies are required by many of the Center's investigators and the Core Facility serves as a central source of numerous monoclonal antibodies. Tissue sections for immunofluorescence staining and for in situ hybridization assays have been necessary to many studies and remain essential to the continuing studies of synovial inflammation. A new addition to the Core Facility is the provision of flow cytometry facilities and expertise in use for phenotypic analysis and separation of lymphoid cells and cultured synovial lining cells or salivary epithelial cells. The principal justifications for this Core facility derive from the highly labor intensive nature of growing and characterizing synovial microenvironment components and the elimination of duplication of effort by individual investigators in obtaining the necessary cell populations and tissues for study. The specific aims of this Core Unit I are to culture synovial cells and to provide fresh samples of macrophages and T cells to investigators for functional studies, to maintain a synovial tissue bank to provide fresh or frozen synovial tissue samples for studies, to provide tissue sections to investigators performing in situ hybridization studies, immunofluorescent assays, or light microscopic analysis, to provide monoclonal antibodies for studies, and to provide flow cytometry facilities and expertise to investigators. This facility is designed to be an important source of tissue culture cells and tissue sections that will be available to all members of the Center.