TISSUE LABORATORY CORE

组织实验室核心

• 批准号: 3810828
• 负责人: KAY H SINGER
• 金额: --
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3810828
• 关键词: T lymphocyte; biomedical facility; biomedical resource; cell bank /registry; histology; human tissue; in situ hybridization; monoclonal antibody; rheumatoid arthritis; sectioning; synovial membrane; tissue /cell culture

Most projects of this SCOR grant will depend to varying degrees upon the availability of cultured and isolated synovial microenvironment components as well as on the availability of fresh and cultured synovial T cell preparations. Moreover, each human tissue, synovial fluid, or peripheral blood specimen will need to be logged in, processed, and in the case of tissue, portions frozen for immunohistology, portions prepared for light microscopy, and portions prepared for electron microscopy. Preparation and use of monoclonal antibodies are also required by many of the Center's investigators. In addition, Project 5 will use the MRL mouse model of arthritis and will require tissue sections for histopathology and in situ hybridization assays, monoclonal antibody technology, and eventually the techniques for growing murine synovial cells. In developing this application, it was decided that the most economical and efficacious approach to meet these needs for the entire program was to centralize these activities. The principal justification for this Core facility derives from the highly labor intensive nature of growing and characterizing synovial microenvironment components and also derives from Dr. Singer's special skills in performing these tasks. The Specific Aims of this Core Unit I are to culture synovial cells and to provide fresh samples of macrophages and T cells to investigators for functional studies, to establish a synovial tissue bank in order to provide fresh or frozen synovial tissue samples for studies, and to provide tissue sections to investigators performing in situ hybridization studies, immunofluorescent assays, or light microscopic analysis. This Core facility is designed to be an important source of tissue culture cells and tissue sections that will be available to all members of the Centers. Use of this facility appropriately will prevent needless duplication of effort and expense and will ensure uniformity of cell populations and tissues studied.

SCOR赠款的大多数项目将在不同程度上取决于 根据培养的和分离的滑膜的可用性， 微环境组件以及 新鲜和培养的滑膜T细胞制品。 而且每 人体组织、滑液或外周血样本将 需要被记录、处理，并且在组织的情况下， 冷冻用于免疫组织学，部分准备用于光照 显微镜检查和制备用于电子显微镜检查的部分。 还需要制备和使用单克隆抗体， 中心的许多调查员。 此外，项目5将使用 MRL小鼠关节炎模型，需要组织切片 对于组织病理学和原位杂交测定， 抗体技术，最终的技术， 小鼠滑膜细胞。 在开发此应用程序时， 决定最经济有效的方法 满足整个计划的这些需求是将这些 活动 核心融资的主要理由是 源于种植业的高度劳动密集性质， 表征滑膜微环境成分， 这是因为辛格博士在执行这些任务时的特殊技能。 本核心单元I的具体目的是培养滑膜细胞 并提供新鲜的巨噬细胞和T细胞样本， 功能研究的研究者，以建立滑膜组织 以提供新鲜或冷冻的滑膜组织样本 用于研究，并向研究者提供组织切片 进行原位杂交研究，免疫荧光 测定或光学显微镜分析。 该核心设施是 被设计为组织培养细胞的重要来源， 组织切片，将提供给所有成员的 中心. 正确使用该设施将防止不必要的 重复劳动和费用，并将确保统一的 研究的细胞群和组织。