Assessing Illumina and Velvet for sequencing a wheat chromosome arm
评估 Illumina 和 Velvet 对小麦染色体臂进行测序
基本信息
- 批准号:BB/G024715/1
- 负责人:
- 金额:$ 5.04万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2009
- 资助国家:英国
- 起止时间:2009 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
World agriculture faces two major and unprecedented challenges in the near future. The first is to expand production of food and feed to meet increased demand while ensuring environmental sustainability. The second is to understand and mitigate the effects of global climate change on food and production. Wheat is one of the world's primary sources of food and feed and is the most important crop by scale and value in Europe. However, wheat yield gains seen in the past 20 years have not been maintained, mainly because germplasm improvement has not continued at sufficient pace to address the major challenges of sustainable food production and adapting to climate change. The new science of genomics can make major contributions to increasing both the scope and speed of crop improvement. But the complexity and size of the wheat genome are major obstacles to establishing biology, breeding and crop improvement strategies based on detailed knowledge of the complete genome sequence. Major advances in genome sequencing methods have led to the development of commercially available instruments that dramatically increase sequence output and reduce costs, and sequence analysis methods have been created to assemble and analyse the sequence generated. However, the strategies and methods required for applying this new technology to the wheat genome remain to be determined. In this proposal we aim to develop methods for sequencing the expressed regions of genes, large insert clones and purified chromosome arms using the Illumina sequencing platform, and to adapt the Velvet sequence assembly software to wheat genomics. The outputs of this project will facilitate the development of cost- effective and efficient strategies for sequencing the complete wheat genome when scaled up. The complete genome sequence of wheat will identify each gene in its correct location on the genomes and will determine the correct structures of genes. This knowledge will be a new foundation for biology projects that aim to understand the functions of wheat genes in processes such as disease resistance, environmental interactions, mineral nutrition and grain yield and nutrition. The methods we aim to develop will also be suitable for re-sequencing wheat lines, for example, commercial varieties that are used in breeding. By comparing these sequences to the reference genome and to each other scientists can identify sequence variation associated with traits. This information can be used to identify genes and lines with desirable genotypes of breeding populations. As such the technologies we aim to develop in this project will be the first step in advancing our capability to breed the next generations of our key crop plant.
在不久的将来,世界农业将面临两大前所未有的挑战。首先是扩大粮食和饲料的生产,以满足日益增长的需求,同时确保环境的可持续性。二是了解和减轻全球气候变化对粮食和生产的影响。小麦是世界上主要的食物和饲料来源之一,在欧洲,就规模和价值而言,小麦是最重要的作物。然而,过去20年小麦产量的增长并没有保持下去,主要原因是种质改良的速度不足以应对可持续粮食生产和适应气候变化的重大挑战。新的基因组学可以为增加作物改良的范围和速度作出重大贡献。但是,小麦基因组的复杂性和大小是建立基于完整基因组序列详细知识的生物学、育种和作物改良策略的主要障碍。基因组测序方法的重大进步导致了商用仪器的发展,这些仪器极大地增加了序列输出并降低了成本,序列分析方法已经被创造出来,用于组装和分析所产生的序列。然而,将这项新技术应用于小麦基因组所需的策略和方法仍有待确定。在本项目中,我们的目标是开发利用Illumina测序平台对基因表达区、大插入克隆和纯化染色体臂进行测序的方法,并使Velvet序列组装软件适应小麦基因组学。该项目的成果将有助于开发成本效益高、效率高的小麦全基因组测序策略。小麦的完整基因组序列将确定每个基因在基因组上的正确位置,并确定基因的正确结构。这些知识将为旨在了解小麦基因在抗病、环境相互作用、矿物质营养、粮食产量和营养等过程中的功能的生物学项目奠定新的基础。我们的目标是开发的方法也将适用于小麦品系的重测序,例如用于育种的商业品种。通过将这些序列与参考基因组和其他序列进行比较,科学家可以确定与性状相关的序列变异。这些信息可用于鉴定育种群体中具有理想基因型的基因和品系。因此,我们在这个项目中开发的技术将是提高我们培育下一代关键作物能力的第一步。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ewan Birney其他文献
Uncovering the heritable components of multimorbidities and disease trajectories using a nationwide cohort
利用全国性队列研究揭示多病症和疾病轨迹的遗传成分
- DOI:
10.1038/s41467-024-51795-8 - 发表时间:
2024-08-28 - 期刊:
- 影响因子:15.700
- 作者:
David Westergaard;Frederik Hytting Jørgensen;Jens Waaben;Alexander Wolfgang Jung;Mette Lademann;Thomas Folkmann Hansen;Jolien Cremers;Sisse Rye Ostrowski;Ole Birger Vesterager Pedersen;Roc Reguant;Isabella Friis Jørgensen;Tom Fitzgerald;Ewan Birney;Karina Banasik;Laust Mortensen;Søren Brunak - 通讯作者:
Søren Brunak
Genomic information infrastructure after the deluge
- DOI:
10.1186/gb-2010-11-7-402 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:9.400
- 作者:
Julian Parkhill;Ewan Birney;Paul Kersey - 通讯作者:
Paul Kersey
Four makes a party
四人成一聚会
- DOI:
10.1038/nature12847 - 发表时间:
2013-12-18 - 期刊:
- 影响因子:48.500
- 作者:
Ewan Birney;Jonathan K. Pritchard - 通讯作者:
Jonathan K. Pritchard
Double Dutch for duplications
双重荷兰语表示重复
- DOI:
10.1038/ng1107-1303 - 发表时间:
2007-11-01 - 期刊:
- 影响因子:29.000
- 作者:
Ewan Birney - 通讯作者:
Ewan Birney
Approaches to comparative sequence analysis: towards a functional view of vertebrate genomes
比较序列分析的方法:迈向脊椎动物基因组的功能观点
- DOI:
10.1038/nrg2185 - 发表时间:
2008-04-01 - 期刊:
- 影响因子:52.000
- 作者:
Elliott H. Margulies;Ewan Birney - 通讯作者:
Ewan Birney
Ewan Birney的其他文献
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{{ truncateString('Ewan Birney', 18)}}的其他基金
The Genetics of Symptom Severity in COVID-19 Infections
COVID-19 感染症状严重程度的遗传学
- 批准号:
BB/V011448/1 - 财政年份:2020
- 资助金额:
$ 5.04万 - 项目类别:
Research Grant
Setting the Standard for Genomics and Health-Related Data Sharing: The Global Alliance for Genomics and Health
制定基因组学和健康相关数据共享标准:全球基因组学与健康联盟
- 批准号:
MC_PC_19024 - 财政年份:2020
- 资助金额:
$ 5.04万 - 项目类别:
Intramural
Towards an international plant genome database
迈向国际植物基因组数据库
- 批准号:
BB/H531519/1 - 财政年份:2010
- 资助金额:
$ 5.04万 - 项目类别:
Research Grant
Pig genome annotation and analysis
猪基因组注释与分析
- 批准号:
BB/E010768/1 - 财政年份:2007
- 资助金额:
$ 5.04万 - 项目类别:
Research Grant
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