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Illumina NovaSeq 6000 Sequencing System

Illumina NovaSeq 6000 测序系统

基本信息

批准号：
10177551
负责人：
Zhao Lai
金额：
$ 60万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-15 至 2022-05-31
项目状态：
已结题

项目摘要

Project Summary: Funds are requested to purchase an Illumina NovaSeq 6000 Sequencing System to upgrade and expand sequencing capacity at the University of Texas Health at San Antonio (UTHSA). The new NovaSeq 6000 will be placed in and managed by the Genome Sequencing Facility (GSF) of Greehey Children’s Cancer Research Institute (GCCRI), a campus-wide shared core facility operated by a highly trained group of staff experienced with Illumina sequencing and genomic technologies and supported by well-qualified team of bioinformaticians. Since its inception in 2011, the GSF has greatly enhanced genomic research capability and fostered scientific collaborations within UTHSA and the greater San Antonio area, carrying out a wide range of sequencing protocols and applications. At the present time, high-throughput sequencing is conducted on a heavily utilized Illumina HiSeq 3000 sequencer that was installed in 2016, and there are no other publicly accessible high- throughput next-generation sequencing platforms within UTHSA or the surrounding San Antonio institutions. The heavy usage of the existing HiSeq 3000 has reached its capacity, and thus the GSF cannot meet the increasing demand for single cell analysis and cost-effective deep sequencing needs of UTHSA investigators and the broader research community. A range of basic, translational, clinical, and genomics studies at UTHSA depend on affordable, customizable high-throughput next-generation sequencing technologies. The requested instrumentation will allow GSF to continue to provide high-quality genomic data to support NIH-funded biomedical research, as shown by the 17 major users and 15 minor users whose research is described in this proposal. UTHSA has demonstrated a strong institutional commitment to the advancement of genomics research and fully supports this grant application by providing matching funds to contribute to continuing operation of the GSF. With the capacity of new requested Illumina NovaSeq 6000 System, a wide variety of research areas will be enhanced, including cancer, aging, pain control, diabetes, immunology, obesity, and neurodegenerative diseases. In addition to the proposed user group, the quality and affordability of high-throughput sequencing data enabled by the NovaSeq 6000 will allow investigators to develop new projects that may take their research into new directions. In addition, this instrumentation will strengthen our educational training programs for pre- and post-doctoral scientists, physicians, and scientists, and foster continued excellence in genomics and bioinformatics research at UTHSA. Thus, acquisition of an Illumina NovaSeq 6000 System will positively impact and expedite a large number of NIH-funded investigations, promote genomic scientific collaborations, and further accelerate translational research to improve the health of those in South Texas, a predominantly Hispanic population.

项目概要：申请资金购买Illumina NovaSeq 6000测序系统进行升级和扩展德克萨斯大学圣安东尼奥分校健康中心 (UTHSA) 的测序能力。新型 NovaSeq 6000 将置于 Greehe 儿童癌症研究中心的基因组测序设施 (GSF) 中并由其管理研究所 (GCCRI) 是一个全校园共享核心设施，由训练有素、经验丰富的员工团队运营借助 Illumina 测序和基因组技术，并得到高素质生物信息学家团队的支持。自2011年成立以来，GSF极大提升了基因组研究能力，培育了科研人员 UTHSA 和大圣安东尼奥地区合作，开展广泛的测序协议和应用程序。目前，高通量测序是在大量使用的 Illumina HiSeq 3000 测序仪于 2016 年安装，目前还没有其他公开可用的高通量测序仪。 UTHSA 或圣安东尼奥周边机构内的高通量下一代测序平台。这现有 HiSeq 3000 的大量使用已达到其容量，因此 GSF 无法满足日益增长的需求 UTHSA 研究人员和研究人员对单细胞分析的需求和具有成本效益的深度测序需求更广泛的研究社区。 UTHSA 的一系列基础、转化、临床和基因组学研究取决于经济实惠、可定制的高通量下一代测序技术。所要求的仪器将使 GSF 能够继续提供高质量的基因组数据来支持 NIH 资助的生物医学研究，如本文描述的 17 个主要用户和 15 个次要用户所示提议。 UTHSA 表现出了对推进基因组学研究的坚定机构承诺并通过提供配套资金全力支持此项赠款申请，以促进该项目的持续运营 GSF。凭借新要求的 Illumina NovaSeq 6000 系统的容量，各种研究领域将得到增强，包括癌症、衰老、疼痛控制、糖尿病、免疫学、肥胖和神经退行性疾病疾病。除了建议的用户群之外，高通量测序的质量和承受能力 NovaSeq 6000 支持的数据将使研究人员能够开发可能需要他们研究的新项目进入新的方向。此外，该仪器将加强我们的预科教育培训计划和博士后科学家、医生和科学家，并培养基因组学和 UTHSA 的生物信息学研究。因此，收购 Illumina NovaSeq 6000 系统将对并加快 NIH 资助的大量研究，促进基因组科学合作，并进一步加速转化研究，以改善以西班牙裔为主的南德克萨斯州人民的健康人口。