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Illumina NovaSeq 6000 Sequencing System

Illumina NovaSeq 6000 测序系统

基本信息

批准号：
10177551
负责人：
Zhao Lai
金额：
$ 60万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-15 至 2022-05-31
项目状态：
已结题

项目摘要

Project Summary: Funds are requested to purchase an Illumina NovaSeq 6000 Sequencing System to upgrade and expand sequencing capacity at the University of Texas Health at San Antonio (UTHSA). The new NovaSeq 6000 will be placed in and managed by the Genome Sequencing Facility (GSF) of Greehey Children’s Cancer Research Institute (GCCRI), a campus-wide shared core facility operated by a highly trained group of staff experienced with Illumina sequencing and genomic technologies and supported by well-qualified team of bioinformaticians. Since its inception in 2011, the GSF has greatly enhanced genomic research capability and fostered scientific collaborations within UTHSA and the greater San Antonio area, carrying out a wide range of sequencing protocols and applications. At the present time, high-throughput sequencing is conducted on a heavily utilized Illumina HiSeq 3000 sequencer that was installed in 2016, and there are no other publicly accessible high- throughput next-generation sequencing platforms within UTHSA or the surrounding San Antonio institutions. The heavy usage of the existing HiSeq 3000 has reached its capacity, and thus the GSF cannot meet the increasing demand for single cell analysis and cost-effective deep sequencing needs of UTHSA investigators and the broader research community. A range of basic, translational, clinical, and genomics studies at UTHSA depend on affordable, customizable high-throughput next-generation sequencing technologies. The requested instrumentation will allow GSF to continue to provide high-quality genomic data to support NIH-funded biomedical research, as shown by the 17 major users and 15 minor users whose research is described in this proposal. UTHSA has demonstrated a strong institutional commitment to the advancement of genomics research and fully supports this grant application by providing matching funds to contribute to continuing operation of the GSF. With the capacity of new requested Illumina NovaSeq 6000 System, a wide variety of research areas will be enhanced, including cancer, aging, pain control, diabetes, immunology, obesity, and neurodegenerative diseases. In addition to the proposed user group, the quality and affordability of high-throughput sequencing data enabled by the NovaSeq 6000 will allow investigators to develop new projects that may take their research into new directions. In addition, this instrumentation will strengthen our educational training programs for pre- and post-doctoral scientists, physicians, and scientists, and foster continued excellence in genomics and bioinformatics research at UTHSA. Thus, acquisition of an Illumina NovaSeq 6000 System will positively impact and expedite a large number of NIH-funded investigations, promote genomic scientific collaborations, and further accelerate translational research to improve the health of those in South Texas, a predominantly Hispanic population.

项目摘要：申请资金用于购买Illumina NovaSeq 6000测序系统，德克萨斯大学圣安东尼奥分校（UTHSA）的测序能力。新的NovaSeq 6000将是由Greehey儿童癌症研究所的基因组测序设施（GSF）管理研究所（GCCRI），一个校园范围内的共享核心设施，由一群训练有素的工作人员，经验丰富，利用Illumina测序和基因组技术，并由合格的生物信息学家团队提供支持。自2011年成立以来，GSF大大增强了基因组研究能力，并促进了科学研究。在UTHSA和大圣安东尼奥地区的合作，进行了广泛的测序协议和应用。目前，高通量测序是在大量使用的 Illumina HiSeq 3000测序仪于2016年安装，并且没有其他可公开访问的高在UTHSA或周围的圣安东尼奥机构内的下一代测序平台。的现有HiSeq 3000的大量使用已经达到其容量，因此GSF无法满足日益增长的 UTHSA研究人员对单细胞分析和具有成本效益的深度测序的需求，更广泛的研究社区。UTHSA的一系列基础，转化，临床和基因组学研究取决于价格实惠、可定制的高通量下一代测序技术。请求的仪器将使GSF继续提供高质量的基因组数据，以支持NIH资助的生物医学研究，如17个主要用户和15个次要用户所示，他们的研究在本报告中有所描述。提议UTHSA已经证明了对基因组学研究进步的强有力的机构承诺并全力支持这项拨款申请，提供配对资金，以促进 GSF。随着新要求的Illumina NovaSeq 6000系统的能力，各种各样的研究领域将包括癌症、衰老、疼痛控制、糖尿病、免疫学、肥胖和神经退行性疾病疾病除了建议的用户群，高通量测序的质量和可负担性 NovaSeq 6000支持的数据将使研究人员能够开发新的项目，新方向此外，这种仪器将加强我们的教育培训计划，和博士后科学家，医生和科学家，并促进在基因组学和生物信息学研究。因此，收购Illumina NovaSeq 6000系统将对加快大量NIH资助的研究，促进基因组科学合作，并进一步加快转化研究，以改善南德克萨斯州的健康，南德克萨斯州主要是西班牙裔，人口