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Microarray bioassays for functional glycome screening

用于功能糖组筛选的微阵列生物测定

基本信息

批准号：
BB/G024820/1
负责人：
Jeremy Turnbull
金额：
$ 14.66万
依托单位：
University of Liverpool
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2009
资助国家：
英国
起止时间：
2009 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG024820%2F1
关键词：
Microarray bioassays functional glycome screening

项目摘要

Complex sugars (glycans) are a highly diverse family of molecules with a broad range of functions in biological processes including cell recognition, adhesion, cell-cell communication and signalling. The study of the glycome - the entire set of glycans expressed by particular cells or tissues - is an emerging field which aims to understand how glycan functions underpin the complexity of human biology, and their involvement in disease processes. The selective interaction of proteins with glycans is the key to their biological functions. A number of new technologies have emerged to support such interaction studies, chief among these being microarray techniques ('glycoarrays'), which allow large numbers of individual glycans to be immobilised on surfaces for examination of their selective binding to protein partners. However, whilst allowing rapid large-scale screening, glycoarrays generate simple interaction data that do not directly reveal functional properties. In this project we propose to develop a novel glycoarray methodology in which live cell responses to large numbers of glycans arrayed on specially modified microscope slides are measured quantitatively. Responses such as cell growth or differentiation will be analysed by fluorescence measurements, using either antibodies or induced expression of fluorescent proteins by the cells themselves. This approach will provide a powerful and generic new tool for decoding the function of the glycome by allowing specific glycan structures to be matched to specific biological functions.

复合糖（聚糖）是一个高度多样化的分子家族，在生物过程中具有广泛的功能，包括细胞识别、粘附、细胞间通信和信号传导。对糖的研究——由特定细胞或组织表达的整套聚糖——是一个新兴领域，旨在了解糖的功能如何支撑人类生物学的复杂性，以及它们在疾病过程中的参与。蛋白质与聚糖的选择性相互作用是其生物学功能的关键。许多新技术已经出现，以支持这种相互作用的研究，其中主要是微阵列技术（“糖阵列”），它允许将大量的单个聚糖固定在表面上，以检查它们与蛋白质伴侣的选择性结合。然而，在允许快速大规模筛选的同时，糖阵列生成的简单相互作用数据并不能直接揭示功能特性。在这个项目中，我们建议开发一种新的糖阵列方法，其中活细胞对特殊修饰的显微镜载玻片上排列的大量聚糖的反应进行定量测量。诸如细胞生长或分化的反应将通过荧光测量来分析，使用抗体或细胞本身诱导的荧光蛋白表达。这种方法将提供一个强大而通用的新工具，通过允许特定的聚糖结构与特定的生物功能相匹配，来解码糖原的功能。