Isolation of pooled selected gene segments for allele discovery by next generation sequencing

通过下一代测序分离汇集的选定基因片段以发现等位基因

• 批准号: BB/G024790/1
• 负责人: Andrew Flavell
• 金额: $ 15.38万
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG024790%2F1
• 关键词: Isolation; pooled; selected; gene; segments

Future crop varieties will need improved performance traits for the consumer and the producer, and reduced environmental impact (e.g. lower fertiliser or pesticide input). These traits are currently monitored by 'molecular markers', which are genetic fingerprinting tests. An alternative approach is to sequence the genetic information directly. A revolution in 'next generation sequencing' is currently underway' with speed doubling annually and prices tumbling, Direct sequencing can give far greater accuracy than molecular markers but there is a technical bottleneck caused by the fact that it sequences many individuals in the same container, raising the difficult issue of how to keep track of which sequence belongs to which individual. In this project we propose to develop, test and compare two promising tracking methods, using barley as a test-bed.

未来的作物品种将需要改善消费者和生产者的表现特征，并减少对环境的影响(例如减少化肥或杀虫剂投入)。这些特征目前由“分子标记”监测，这是一种遗传指纹测试。另一种方法是直接对遗传信息进行排序。“下一代测序”目前正在进行一场革命，速度每年翻一番，价格暴跌，直接测序可以比分子标记提供更高的准确性，但存在一个技术瓶颈，这是因为它在同一容器中对许多个体进行测序，这引发了如何跟踪哪个序列属于哪个个体的难题。在这个项目中，我们打算以大麦为试验床，开发、测试和比较两种有前景的跟踪方法。