ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE

本项目的目标之一是研究细胞的超微结构。 甲壳类伸展感受器与其功能的关系。我们计划 研究牵张引起的感觉树突的结构变化 产生电位的神经元，通过使用超快速冷冻和 冷冻替换法。此外，膜的精细结构 受体神经元上的抑制性突触将用 冷冻断裂法。这个项目的另一个目标是调查 新形成细胞的超微结构和电生理特性 组织培养中的神经肌肉连接。我们还将研究发展 细胞外乙酰胆碱受体的研究 显微电生理和冷冻断裂法。

One of the objectives of this project is to investigate the ultrastructure of the crustacean stretch receptor organ in relation to its function. We plan to investigate stretch-induced structural changes in the dendrities of the sensory neuron, where the generator potential occurs, by using ultraquick freezing and freeze-substitution methods. In addition, the membrane fine structure of the inhibitory synapses on the receptor neuron will be studied by using the freeze-fracture method. The other objective of this project is to investigate the ultrastructural and electrophysiological properties of newly-formed neuromuscular junctions in tissue culture. Also we will study the development of extrajunctional acetylcholine receptors in cultured muscle cells by using microelectrophysiological and freeze-fracture methods.