ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
批准号:
2330184
负责人:
YASUKO NAKAJIMA
金额:
$20.5万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-03-01 至 2001-01-31
关键词:
G protein acetylcholine antisense nucleic acid biological signal transduction corticotropin releasing factor electrophysiology immunocytochemistry laboratory rat locus coeruleus microinjections neural information processing neural transmission neurons neurophysiology neurotensin norepinephrine phosphoprotein phosphatase potassium channel protein kinase receptor coupling second messengers somatostatin substance P tissue /cell culture voltage /patch clamp
中文摘要
描述:大脑中每个神经元的活动受到
英文摘要
DESCRIPTION: The activity of each neuron in the brain is influenced by
the actions of many "slow" neurotransmitters constantly arriving from
other neurons, and the total sum of these actions determines the
excitability of the neuron. The main objective of this grant proposal is
to elucidate the physiological mechanisms by which "slow" excitatory and
"slow" inhibitory peptide transmitters produce their actions on brain
neurons. Cholinergic neurons from the nucleus basalis of Meynert and
noradrenergic neurons from the locus coeruleus in culture prepared from
the rat brain will be used; the technique for culturing these neurons was
first developed in the PI's laboratory in 1985. These neurons are the
main source of acetylcholine and noradrenaline in the brain, and in
humans these neurons show degeneration in Alzheimer's disease. The
projects focus on the signal transduction mechanisms of the following
transmitters: substance P, neurotensin, corticotropin-releasing factor,
and somatostatin. Some of these transmitters have been reported to be
reduced in Alzheimer's disease.
Particular emphasis will be placed on how these transmitters modulate the
activity of the inward rectifier K+ channel. In many brain neurons, the
modulation of the inward rectifier K+channel is responsible for the
generation of slow excitatory and slow inhibitory synaptic potentials.
The specific projects are: (1) to identify which G protein mediates the
signal transduction of the effects of substance P, neurotensin,
somatostatin, and corticotropin-releasing factor on the activity of the
K+ channels; (2) to investigate the roles of second massagers
(phospholipase C and protein kinase C, cyclic AMP and cyclic AMP-
dependent protein kinase, and protein phosphatase) in the signal
transduction of these transmitters, and (2) to investigate the mechanisms
of the interaction of G protein subunits (mutated and non-mutated),
protein kinase, and phosphatases with the inward rectifier K+ channels
by using inside-out patches. Patch-clamp electrophysiology and
immunocytochemistry will be used. In addition, a microinjector will be
used to preform intracellular injection of peptides, antibodies, and
antisense DNA in order to disrupt the function of a particular mediator
of the signal transduction cascades.
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NEUROPHARMACOLOGY OF AROUSAL AND SLEEP DISORDERS
-
批准号:6847995
-
项目类别:
-
资助金额:$33.32万
-
财政年份:2003
-
负责人:YASUKO NAKAJIMA
-
依托单位:
NEUROPHARMACOLOGY OF AROUSAL AND SLEEP DISORDERS
-
批准号:6702334
-
项目类别:
-
资助金额:$33.32万
-
财政年份:2003
-
负责人:YASUKO NAKAJIMA
-
依托单位:
NEUROPHARMACOLOGY OF AROUSAL AND SLEEP DISORDERS
-
批准号:6614324
-
项目类别:
-
资助金额:$35.69万
-
财政年份:2003
-
负责人:YASUKO NAKAJIMA
-
依托单位:
NEUROPHARMACOLOGY OF AROUSAL AND SLEEP DISORDERS
-
批准号:7047864
-
项目类别:
-
资助金额:$32.53万
-
财政年份:2003
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:2871439
-
项目类别:
-
资助金额:$21.84万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:3116892
-
项目类别:
-
资助金额:$15.94万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:3116891
-
项目类别:
-
资助金额:$15.69万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:6847748
-
项目类别:
-
资助金额:$31.17万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:2049445
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项目类别:
-
资助金额:$20.52万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:3116893
-
项目类别:
-
资助金额:$18.35万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:3116887
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项目类别:
-
资助金额:$18.18万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:6574523
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项目类别:
-
资助金额:$33.67万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
OPOID EFFECTS ON SUBSTANTIA NIGRA AND OTHER NEURONS
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批准号:3212205
-
项目类别:
-
资助金额:$12.83万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:2653719
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项目类别:
-
资助金额:$21.0万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:3116894
-
项目类别:
-
资助金额:$18.74万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:6437158
-
项目类别:
-
资助金额:$10.58万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
OPOID EFFECTS ON SUBSTANTIA NIGRA AND OTHER NEURONS
-
批准号:3212207
-
项目类别:
-
资助金额:$14.32万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:6149909
-
项目类别:
-
资助金额:$22.71万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:7214667
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项目类别:
-
资助金额:$29.56万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE
-
批准号:7014529
-
项目类别:
-
资助金额:$30.44万
-
财政年份:1988
-
负责人:YASUKO NAKAJIMA
-
依托单位:
海外基金