ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE

神经和肌肉的超微结构和功能

• 批准号: 3116891
• 负责人: YASUKO NAKAJIMA
• 金额: $ 15.69万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3116891
• 关键词: Crustacea; acetylcholine; electron microscopy; electrophysiology; embryo /fetus; freeze etching; fresh water environment; histochemistry /cytochemistry; membrane proteins; neural information processing; neuroanatomy; neuromuscular junction; neuromuscular transmission; neurophysiology; nicotinic receptors; stretch receptors; synapses; tissue /cell culture

One of the objectives of this project is to investigate the ultrastructure of the crustacean stretch receptor organ in relation to its function. We plan to investigate stretch-induced structural changes in the dendrities of the sensory neuron, where the generator potential occurs, by using ultraquick freezing and freeze-substitution methods. In addition, the membrane fine structure of the inhibitory synapses on the receptor neuron will be studied by using the freeze-fracture method. The other objective of this project is to investigate the ultrastructural and electrophysiological properties of newly-formed neuromuscular junctions in tissue culture. Also we will study the development of extrajunctional acetylcholine receptors in cultured muscle cells by using microelectrophysiological and freeze-fracture methods.

本项目的目的之一是研究 甲壳类动物的伸展感受器官与其功能的关系。 我们计划 研究感觉神经树突中拉伸引起的结构变化 神经元，其中发生器电位，通过使用超快速冷冻， 冷冻置换法。 此外，膜的精细结构， 受体神经元上的抑制性突触将通过使用 冷冻断裂法 该项目的另一个目标是调查 新形成的细胞的超微结构和电生理特性 组织培养中的神经肌肉接头。 我们还将研究 的连接外乙酰胆碱受体在培养的肌肉细胞， 微电生理学和冷冻断裂法。