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IMMORTALIZATION OF SV40-TRANSFORMED HUMAN CELLS

IMMORTALIZATION OF SV40-TRANSFORMED HUMAN CELLS
SV40 转化人类细胞的永生化
批准号:
3115384
负责人:
HARVEY L OZER
金额:
$27.8万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-12-01 至 1992-08-31

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中文摘要
翻译
来源于正常组织的人类二倍体细胞具有一个特征
英文摘要
Human diploid cells derived from normal tissue undergo a characteristic pattern of growth in cell culture, ending with a non-replicative phase and, finally, cell death. Models of cellular aging have been developed which suggest analogy between this pattern and behavior of the organism in vivo; hence, the term "senescence". Human diploid fibroblasts (HF) which had been infected in vitro by the oncogenic DNA virus SV40 have been found to overcome this phenomenon. We have found that a clonal isolate of a HF transformed by an origin-defective mutant of SV40 (SVori-) with an intact early region permits isolation of immortalized cells at an unusually high frequency. Analysis of several such immortalized HF from individual cloned transformants will be performed to identify possible common genetic alterations. Further, we propose to study a series of independent HF transformed by origin-defective mutants of SV40 with a temperature-sensitive T antigen (ts A mutants) to assess possible bases for the role of SV40 in the initiation and maintenance of a continuous cell strain.
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Interdisciplinary Training Program in Cancer Research
Interdisciplinary Training Program in Cancer Research
Interdisciplinary Training Program in Cancer Research
  • 批准号:
    8701009
  • 项目类别:
  • 资助金额:
    $6.57万
  • 财政年份:
    2010
  • 负责人:
    HARVEY L OZER
  • 依托单位:
Interdisciplinary Training Program in Cancer Research
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