GENETICS OF LAMBDA PROPHAGE - BIOTIN OPERON

Lambda 原噬菌体的遗传学 - 生物素操纵子

• 批准号: 3124422
• 负责人: ALLAN M CAMPBELL
• 金额: $ 30.37万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124422
• 关键词: bacteriophage lambda; prophages; virus genetics

The DNA base sequence of the pI promoter of the lambda-related phage 21 will be determined. We have already sequenced pI from phage 434. Phage 21 is of interest because, unlike 434, it has a different insertion site than lambda. However, its pI promoter responds to the cII protein. Mutations in the pI promoter of lambda will be identified and sequenced. The structural gene for biotin sulfoxide reductase (bisC) will be cloned on a multi-copy plasmid. This should provide us with sufficient bisC protein to obtain a pure product, as well as facilitating genetic analysis. Insertions of Mu Ap 1ac in bisC will be selected so that the regulation, if any, of bisC can be examined. We have found that this enzyme uses the same molybdenum cofactor (product of genes ch1A, ch1B, ch1E, ch1G) as nitrate reductase. The relation of those genes to bisC regulation will be tested. The birA gene, structural gene for the bifunctional protein holoenzyme-synthetase biotin-repressor, already cloned as a 2.1 kb segment, will be sequenced in wild type and mutant form.

噬菌体相关噬菌体21的pI启动子的DNA碱基序列将是 测定 我们已经对来自噬菌体434的pI进行了测序。 噬菌体21是 因为与434不同，它具有与λ不同插入位点。 然而，其pI启动子响应cII蛋白。 pI突变 将鉴定λ的启动子并测序。 生物素亚砜还原酶（bisC）的结构基因将克隆在一个 多拷贝质粒。 这将为我们提供足够的bisC蛋白， 获得纯的产物，以及便于遗传分析。 的插入 将选择BisC中的Mu Ap 1ac，以便BisC的调节（如果有的话）可以 接受检查。 我们发现这种酶使用相同的钼辅因子 （基因ch 1A、ch 1B、ch 1E、ch 1G的产物）作为硝酸还原酶。 的关系 这些基因对bisC的调控将被测试。 birA基因，双功能蛋白的结构基因 全酶合成酶生物素阻遏物已经克隆为2.1kb片段， 以野生型和突变体形式进行测序。