GENETICS OF LAMBDA PROPHAGE - BIOTIN OPERON

Lambda相关噬菌体21的PI启动子的DNA碱基序列为 下定决心。我们已经对噬菌体434的等电点进行了测序。噬菌体21是 之所以感兴趣，是因为与434不同的是，它的插入位点与lambda不同。 然而，它的PI启动子对CII蛋白有反应。猪的基因突变 将对lambda的启动子进行鉴定和测序。 生物素亚砜还原酶(BSC)结构基因将被克隆到 多拷贝质粒。这应该会为我们提供足够的BSC蛋白 获得纯净的产品，以及促进基因分析。插入的内容 将选择BSC中的MU AP 1AC，以便BSC的法规(如果有)可以 接受检查。我们发现这种酶使用相同的钼辅因子 (ch1A、ch1B、ch1E、ch1G基因产物)为硝酸还原酶。与中国的关系 这些对Bisc调控的基因将被测试。 BIRA基因，双功能蛋白的结构基因 全酶合成酶生物素抑制物，已经被克隆为2.1kb的片段，将 以野生型和突变型的形式进行测序。

The DNA base sequence of the pI promoter of the lambda-related phage 21 will be determined. We have already sequenced pI from phage 434. Phage 21 is of interest because, unlike 434, it has a different insertion site than lambda. However, its pI promoter responds to the cII protein. Mutations in the pI promoter of lambda will be identified and sequenced. The structural gene for biotin sulfoxide reductase (bisC) will be cloned on a multi-copy plasmid. This should provide us with sufficient bisC protein to obtain a pure product, as well as facilitating genetic analysis. Insertions of Mu Ap 1ac in bisC will be selected so that the regulation, if any, of bisC can be examined. We have found that this enzyme uses the same molybdenum cofactor (product of genes ch1A, ch1B, ch1E, ch1G) as nitrate reductase. The relation of those genes to bisC regulation will be tested. The birA gene, structural gene for the bifunctional protein holoenzyme-synthetase biotin-repressor, already cloned as a 2.1 kb segment, will be sequenced in wild type and mutant form.