GENETICS OF LAMBDOID PROPHAGES

兰布科原噬菌体的遗传学

• 批准号: 2189419
• 负责人: ALLAN M CAMPBELL
• 金额: $ 28万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2189419
• 关键词: DNA topoisomerases; Escherichia coli; bacteriophage lambda; biotin; gene complementation; gene induction /repression; gene mutation; genetic manipulation; integrase; lysogeny; open reading frames; operon; oxidoreductase; prophages; sulfoxide; virus DNA; virus genetics

The long term goals are to understand how viruses interact with chromosomes and how genes for biosynthesis of proteins needed in small amounts are regulated. The health relatedness of these studies on prokaryotic model systems lies in the elucidation of basic mechanisms and in the perspective shed on natural biology of disease-causing viruses by the common features of bacterial and animal virology. The DNA of bacteriophage lambda upstream of the integrase operon will be examined for effects on integration and excision of two elements: a terminator-like sequence tI' that abuts the pI promoter and an open reading frame (ORF-55) upstream from it. Phage constructed to eliminate tI' will be examined for the effect of cII protein in the presence of transcription from pL, to test the hypothesis that tI' functions in the initial transition from the uncommitted to the lysogenic state. The ORF-55 gene expressed from a plasmid will be tested for complementation of the excisionase defect of the lambda crg phage, which has an IS2 insertion in ORF-55. Lambda crg prophages in inverted orientation will be tested for the ability to generate duplications by recombination with the defective gsr' prophage, and the defective prophages of E. coli strains of the ECOR collection will be compared. The properties of purified E. coli biotin sulfoxide reductase and the molecular basis for a newly evolved BDS reductase activity will be determined.

长期目标是了解病毒如何与 染色体和基因如何生物合成所需的蛋白质， 小部分是受管制的。 这些疾病的健康相关性 对原核生物模型系统的研究在于阐明 基本机制和自然生物学的观点 致病病毒的共同特征， 动物病毒学 λ噬菌体上游的DNA 将检查整合酶操纵子的作用， 两个元件的整合和切除：终止子样 邻接pI启动子和开放阅读框的序列tI （ORF-55）上游。 将检查cII蛋白在以下存在下的作用： 从pL转录，以测试假设，tI'的功能， 从未定型到溶原状态的初始转变。 将检测从质粒表达的ORF-55基因， λ CRG的切除酶缺陷的互补 噬菌体，其在ORF-55中具有IS 2插入。 λ crg 将测试反向原噬菌体的能力， 通过与有缺陷的GSR重组产生复制 原噬菌体和E.大肠杆菌菌株 将比较ECOR收集。 纯化的E. 大肠杆菌生物素亚砜还原酶及其新的 将测定进化的BDS还原酶活性。