Investigating the role of a kinesin gene in butterfly mimicry

研究驱动蛋白基因在蝴蝶拟态中的作用

• 批准号: BB/H014357/1
• 负责人: Mark Blaxter
• 金额: $ 22.42万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH014357%2F1
• 关键词: Investigating; role; kinesin; gene; butterfly

Mimicry among butterfly species is a classic example of evolution and adaptation. The brightly coloured neotropical Heliconius butterflies are one of the best studied examples, but the molecular genetic basis of mimicry remains poorly understood. In particular mimicry offers an opportunity to study the repeatability of evolution, as the same patterns emerge again and again in divergent lineages. The project falls into two broad areas, first we use cutting edge sequencing technology to make a genetic map of the H. melpomene genome. This will be used to help assemble the genome sequence currently being generated. This will form the basis for a genome-wide survey of adaptive divergence between H. melpomene races. Divergent geographic populations of this species form narrow hybrid zones where they hybridise and exchange genes. Thus, narrow regions of the genome controlling wing patterns are genetically differentiated against a background of extensive recombination. This offers a powerful opportunity to identify changes responsible for wing pattern differentiation. We will first characterise variation within and between races by genome resequencing at low coverage. Then we will use novel 'sequence capture' technology to enrich genomic DNA for regions of interest from 96 individuals, taken from six phenotypic races of H. melpomene. The experiment will be designed to sample two chromosomal regions containing wing patterning genes, and a further 12,000 variable sites located across the genome. This will offer a unique genome-wide analysis of parallel divergence between the six populations sampled. In particular we aim to determine a) how much of the genome is involved in colour pattern divergence b) whether the same regions are implicated across independent hybrid zones c) estimate the age of the alleles involved in wing pattern divergence and d) identify putative functional sites for further analysis. The second major aim of the project is to investigate the kinesin gene that represents a strong candidate locus for controlling the red forewing band of H. melpomene. We will study the spatial distribution of kinesin gene expression patterns between divergent phenotypes, in order to test whether spatial regulation underlies pattern regulation. Many genes show different variants generated by alternative splicing, generating variant forms of the protein containing alternative forms of the exons. Alternative splicing is a potentially powerful but under-explored mechanism that could generate evolutionarily relevant variation. We have evidence for alternative splicing the Kinesin gene, and here will characterise the isoforms of this gene and test for correlations between isoform expression and wing phenotype. We will investigate the molecular function of the gene, including a search for other molecules that interact with the kinesin protein, and test to confirm its motor function. Finally, we will develop trangenics methods for explicitly testing the function of the kinesin gene in wing pattern specification in divergent races of H. melpomene. The major gene dominant control of the red band means that we expect to be able to generate a red-banded phenotype by expressing the 'red' kinesin allele in a yellow banded phenotype. This will provide the first explicit test of function for a gene causing pattern variation in any butterfly.

蝴蝶物种的拟态是进化和适应的经典例子。色彩鲜艳的新热带Heliconius蝴蝶是研究得最好的例子之一，但模仿的分子遗传基础仍然知之甚少。特别是模仿提供了一个研究进化可重复性的机会，因为相同的模式在不同的谱系中一次又一次地出现。该项目福尔斯两大领域，首先，我们使用最先进的测序技术来制作H。melpomene基因组。这将用于帮助组装目前正在生成的基因组序列。这将为在全基因组范围内调查H。melpomene种族。本种的分歧的地理种群形成狭窄的杂交带，在那里它们杂交和交换基因。因此，控制翼模式的基因组的狭窄区域在广泛重组的背景下遗传分化。这提供了一个强有力的机会，以确定负责翼型分化的变化。我们将首先通过低覆盖率的基因组重测序来检测种族内和种族间的变异。然后，我们将使用新的“序列捕获”技术，从96个个体的基因组DNA中富集感兴趣的区域，这些个体来自6个表型的H。美波美该实验将被设计为对两个含有翅膀图案基因的染色体区域进行采样，以及位于整个基因组中的另外12，000个可变位点。这将提供一个独特的全基因组分析的平行分歧之间的六个人口抽样。特别是，我们的目标是确定a）基因组中有多少参与颜色模式分歧B）是否在独立的杂交区中涉及相同的区域，c）估计参与翅膀模式分歧的等位基因的年龄，以及d）鉴定用于进一步分析的推定的功能位点。该项目的第二个主要目的是研究驱动蛋白基因，该基因是控制H.美波美我们将研究不同表型之间驱动蛋白基因表达模式的空间分布，以检验空间调控是否是模式调控的基础。许多基因显示出通过选择性剪接产生的不同变体，从而产生含有外显子的选择性形式的蛋白质的变体形式。选择性剪接是一种潜在的强大但尚未开发的机制，可以产生进化相关的变异。我们有选择性剪接驱动蛋白基因的证据，这里将展示该基因的异构体，并测试异构体表达和翅膀表型之间的相关性。我们将研究该基因的分子功能，包括寻找与驱动蛋白相互作用的其他分子，并测试以确认其运动功能。最后，我们将发展转基因学方法，明确测试驱动蛋白基因的功能，在翅型规格在不同种族的H。美波美红带的主基因显性控制意味着我们期望能够通过在黄带表型中表达“红色”驱动蛋白等位基因来产生红带表型。这将提供第一个明确的测试功能的基因导致模式的变化，在任何蝴蝶。

项目成果

Genome-wide patterns of divergence and gene flow across a butterfly radiation

蝴蝶辐射中的全基因组分歧和基因流模式

• DOI: 10.1111/j.1365-294x.2012.05730.x
• 发表时间: 2012
• 期刊: Molecular Ecology
• 影响因子: 4.9
• 作者: Nadeau N

Eyespot variation and field temperature in the Meadow Brown butterfly.

• DOI: 10.1002/ece3.10842
• 发表时间: 2024-01
• 期刊: ECOLOGY AND EVOLUTION
• 影响因子: 2.6
• 作者: Mowbray, Sophie;Bennie, Jonathan;Rhodes, Marcus W.;Smith, David A. S.;ffrench-Constant, Richard H.
• 通讯作者: ffrench-Constant, Richard H.

Genome-wide evidence for speciation with gene flow in Heliconius butterflies.

• DOI: 10.1101/gr.159426.113
• 发表时间: 2013-11
• 期刊: Genome research
• 影响因子: 7
• 作者: Martin SH;Dasmahapatra KK;Nadeau NJ;Salazar C;Walters JR;Simpson F;Blaxter M;Manica A;Mallet J;Jiggins CD
• 通讯作者: Jiggins CD

Butterfly genome reveals promiscuous exchange of mimicry adaptations among species.

• DOI: 10.1038/nature11041
• 发表时间: 2012-07-05
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Dasmahapatra, Kanchon K.;Walters, James R.;Briscoe, Adriana D.;Davey, John W.;Whibley, Annabel;Nadeau, Nicola J.;Zimin, Aleksey V.;Hughes, Daniel S. T.;Ferguson, Laura C.;Martin, Simon H.;Salazar, Camilo;Lewis, James J.;Adler, Sebastian;Ahn, Seung-Joon;Baker, Dean A.;Baxter, Simon W.;Chamberlain, Nicola L.;Chauhan, Ritika;Counterman, Brian A.;Dalmay, Tamas;Gilbert, Lawrence E.;Gordon, Karl;Heckel, David G.;Hines, Heather M.;Hoff, Katharina J.;Holland, Peter W. H.;Jacquin-Joly, Emmanuelle;Jiggins, Francis M.;Jones, Robert T.;Kapan, Durrell D.;Kersey, Paul;Lamas, Gerardo;Lawson, Daniel;Mapleson, Daniel;Maroja, Luana S.;Martin, Arnaud;Moxon, Simon;Palmer, William J.;Papa, Riccardo;Papanicolaou, Alexie;Pauchet, Yannick;Ray, David A.;Rosser, Neil;Salzberg, Steven L.;Supple, Megan A.;Surridge, Alison;Tenger-Trolander, Ayse;Vogel, Heiko;Wilkinson, Paul A.;Wilson, Derek;Yorke, James A.;Yuan, Furong;Balmuth, Alexi L.;Eland, Cathlene;Gharbi, Karim;Thomson, Marian;Gibbs, Richard A.;Han, Yi;Jayaseelan, Joy C.;Kovar, Christie;Mathew, Tittu;Muzny, Donna M.;Ongeri, Fiona;Pu, Ling-Ling;Qu, Jiaxin;Thornton, Rebecca L.;Worley, Kim C.;Wu, Yuan-Qing;Linares, Mauricio;Blaxter, Mark L.;Ffrench-Constant, Richard H.;Joron, Mathieu;Kronforst, Marcus R.;Mullen, Sean P.;Reed, Robert D.;Scherer, Steven E.;Richards, Stephen;Mallet, James;McMillan, W. Owen;Jiggins, Chris D.
• 通讯作者: Jiggins, Chris D.