MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS

将试图回答几个问题的性质， 呼肠孤病毒基因组中编码的功能和关于呼肠孤病毒的功能 乘法 它们是：a.呼肠孤病毒的形态发生将在 体内通过分析RNA和蛋白质成分的性质， 逐渐更成熟的未成熟呼肠孤病毒颗粒，从 第一个复合物，其中十种单独的ssRNA分子 组装好了 它也将在体外进行研究， 天然呼肠孤病毒蛋白与ssRNA分子的组合和分离 并表征所产生的复合物。 B. 天然呼肠孤病毒蛋白 将被检查是否拥有五种酶活性， 由呼肠孤病毒核心展示。 这种蛋白质将从细菌中分离出来 通过表达载体转化，其中呼肠孤病毒基因已经 克隆的 C. 试图阐明呼肠孤病毒的功能 通过详细检查ts突变体的增殖周期， 不允许的温度 十个基因中每一个都有病变的突变体 都是可用的。 倍增周期将根据以下方面进行分析： ss和dsRNA种类和呼肠孤病毒蛋白的合成动力学，如 以及呼肠孤病毒形态发生途径上的各种颗粒。 D. 各种呼肠孤病毒信使RNA种类不被翻译 同等效率。 上游和周边地区的重要性 起始密码子作为控制翻译效率的元件， 通过引入突变、缺失和插入进行研究。 是. 三种呼肠孤病毒的进化和功能关系 血清型将通过对其基因的同源组进行测序来研究， 检查它们蛋白质的同源集合，以确定三级结构的相似性。 结构以及它们的抗原和功能结构域的性质。 F. 利巴韦林抗呼肠孤病毒活性的模式，以及各种纯 干扰素种类将通过确定干扰素的哪些反应来检查。 它们抑制呼肠孤病毒增殖周期。 希望这些结果能 可以扩展并应用于轮状病毒和环状病毒的感染， 医学重要性

Attempts will be made to answer several questions concerning the nature of the functions encoded in the reovirus genome and concerning reovirus multiplication. They are: a. Reovirus morphogenesis will be estudied in vivo by analyzing the nature of the RNA and protein components of progressively more mature immature reovirus particles, starting with the first complexes into which the ten individual species of ssRNA molecules are assembled. It will also be studied in vitro by reacting various combinations of native reovirus proteins with ssRNA molecules and isolating and characterizing the complexes that result. b. Native reovirus proteins will be examined for possession of the five enzyme activities that are exhibited by reovirus cores. Such proteins will be isolated from bacteria transformed by expression vectors into which reovirus genes have been cloned. c. Attempts will be made to elucidate the functions of reovirus proteins by examining in detail the multiplication cycles of ts mutants at nonpermissive temperatures. Mutants with lesions in each of the ten genes are available. Multiplication cycles will be analyzed in terms of the kinetics of synthesis of ss and dsRNA species and reovirus proteins, as well as of the various particles on the pathway of reovirus morphogenesis. d. The various reovirus messenger RNA species are not translated with equal efficiency. The importance of regions upstream from and around initiation codons as elements that control efficiency of translation will e investigated by introducing mutations, deletions and insertions into them. be. The evolutionary and functional relationships of the three reovirus serotypes will be studied by sequencing cognate sets of their genes and examining cognate sets of their proteins for similarity of tertiary structure and for the nature of their antigenic and functional domains. f. The mode of the anti-reovirus activity of ribavirin, and various pure interferon species will be examined by determining which reactions of the reovirus multiplication cycle they inhibit. Hopefully, these results can be extended and applied to infections by rotaviruses and orbiviruses of medical importance.